Aniseed - Construct

Molecular Tool 'Mesp>RapS17N' >
Molecular Tool 'Raf-3xFLAGtag mRNA (Tokuoka 2018)' >
Molecular Tool 'Beta-catenin MO (Wada 2008)' >
Construct 'CONS00000194' >
Construct 'CONS00001499'

Construct

ID

CONS00001499

Name

pFoxF -781bp/64bp::H2B>mCh

Min Promoter

Endogenous Promoter

Reporter

mCherry

Comment

Construct containing the FoxF epidermal enhancer driving mCherry expression by using H2B:mCherry, a fusion protein for nuclear localisation.

This construct is testing Regulatory Region

REG00000196: FoxF -781/64

Sequence

Show sequence

Length: 845

CGTTCGAGAGAGCAGCGCTTTAATTAATTACCGAGGTGGTCGCCGCTCCGCCGCGGGCGA
GGTAGCAATTATACTTAATAAAGAGCCGTTAAGAAGTAGCAACTTTCGCCATTCAGTCAG
TCGTCGGCGCAGTAAATGTGTAGCTTCGTTTATGGGGATTATTCACGAATTAAGTCAACA
AGTTTATAGCAAAATTACGAGTCGTAAAGGTTGCCGATTCTATTCGAGGTATTTCGGTTT
ACAAGCCATGCCCCTTTACGGCGTTTTGGTAAAACTTAAACTAAAATAAAAATATGCTTA
AAGTTATCCTTAGACAAAAATCACGAGTTTGTACTAAATATAACATATACATATAAAATA
TAAGGAGATATATTTTTGTCGTGCCAGTTTCACACGATCAAGGCGTGTTAAAAATAAATA
CAGCGACTAGATTTAGAACTTGGAGCTATTCTTACTGAGCCATCTTGGATTGTTTATTTT
AAAATACAGACAGAGTTTATTTTGAAAGTAAACTTGGAGAAACGTTTGTAGCATCAAGAC
GTGATAGACAGAACTGTTTACTACGCGCTGAAGTTGTTTAATGTTTGAGCGATTCGGTTT
GTTTGCTGTTTATAAAACTCTGATGTAGCGGTTGGCCTAAGACTGTTTTGTTTGCCTGAT
GTAATCTGTCTGCTATTTAAATAAGAACAGAGAAAGCAAGCGTTCTTATTTTTGCTAAAT
ACAAAATATTTTATTAAGTATTTAGGAGTATTTATAATTTTAACCTGTGTTCTTTATTTA
AAAGATTTCTTTTAAGCGCTTTTAAATTATTACAAGACCAGTTTTACAACATCAGTTATT
GAAGT

Reference(s)

Gline et al. (2015). Surrounding tissues canalize motile cardiopharyngeal progenitors towards collective polarity and directed migration. Development 142(3):544-54

Show in situ data