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CONSTRUCT

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  1. Construct 'CONS00001525'

Construct

ID

CONS00001525

Name

p.Cirobu.REG.KhC6.1500090-1502346>unc-76::venus

Min Promoter

Cirobu.REG.KhC6.1502506-1503107|Neurogenin

Reporter

venus

Comment

Neurogenin b line enhancer drives venus expression in the bipolar tail neuron precursors. Caenorhabditis elegans UNC-76 tags was fused to fluorescent protein to ensure even labelling of axons.

This construct is testing Regulatory Region

REG00001496: Cirobu.REG.KhC6.1500090-1502346|Neurogenin

Sequence

Show sequence

Length: 2,257

GTCTGTTTCCGCATACATGCAATATGCACGCGTTCATTATAATATCAAGTTTTACCAATT
TCCGTAAATTTTTAATGGAACAGTGTAATTAACCCGACTATATTACCGTTTTCCGATCGC
ATTGTTACGTCACAATAAAACCGCGGTGTGTTCTGCGAAGTTAGGAAGTATCTCATTACG
TCATAATCGTCGGATAGTTTTGATTGTTTTATCATAATGCGGACTAAGTAGTCCATTACT
ACACCGACGTAATCGCCACTTTGTGACGTCACAATACCGCACCAGATCAATGACGTCATC
AAAGGGGAAACTCGGAAGTAATAAGTGACGAAATCAAAGGCGGAATTATGACGTTTTCGC
TTTTGATTGTTACGTACGTGGGAAAGAGCAAACGAATCGGGAAACGTTTTGCTATCGTAG
ATTGTGACGAAATAATGAAATAGATTGTGACGTCATGATGATTTTTATCTCCGAATAAAC
GGAATCGAGTTATCAGCACACGCGTCACGAACATTGTTTACCAACTGAATACAATTGTTA
AGTTATTTAAATAAATTTTAATCACCTTAAACCGAGCTGAATTACACGCCGATCAAACAT
ATATTATTTGTTTTACTTTGTCGTTACATTTGTACTTGCTAAAGCAAATTCGCTTTAAAA
AACGGCCCTTCTGAAAGCTTTGACTGGTAAATGACATCAAAGCTGCGGCGTTTAAAAGCT
GTTTACTGTTCGTTGTTTAATCGGTACAGTGTTTGGTGTTTATTCTATACTCCAGTCTGG
TTTATTTTATTTATTTTACACCGTTTTGGTTAATTTTGTTGCGTAATTTAAAATCTGGTA
CACTGTTTGTCCCGTATTTCCCTACCCGCATTACGAGACCTGATTTTACGCTGTGTGACG
TCACGCCATATGGCACAAAATGCAACTTTAAACGAATAAGCAGCGTCCACAAAATACATA
TCGGGTGTATTGCGTCGCGCTAATTGCTCAGATTTCCGGAAGTATAATATTTCAACCAAT
CTCTGCTTATTAAAACGTTAGATTGACTAATTAGAAACCACGAATAATGTTAATTGCTCC
AACCCAGTGGTTCATTAATTATGGGTTGTCTAAAATAAATGTTGGCCAACACATTTCAGA
AAATTAAGAATTCCAAAGTGTTAATCACCCACAAAGTAACATACGTAGTAGCTCGTAAGC
GGGCACGAGGTGTATGAAACAGAACACCCGTGTTATAACAACTGCCATCATAGATATCTA
AACATATGTTTAGATATCTATGCTGCCATTACTCCGCAATGCGCGGATAAATAAATTACA
TTCATTCGTATTTGCAAAAATGGTGTTTTTATGTCTGTACGGTCCGGGCATAAAGACGAC
AGATTGCCACGCATGCAGTCGGGATCAATTATTTATAAATAATATGAATTATTCACGAGG
AATGGCGCTTTCCTGAGCCCCGTGATTTAGGAAAATTTGAGAAAAATCACAAAAATAAAT
TACGAAGTCGAAAATGGAGAAATTTTGGGTAATTTTTATCATGAAATATGACATGCGTCC
AAATTCGTGTATAGTAGGTTGGGGTAAAGTGGTCCATGTTTTTATTCTATTTGTCGTCTC
ATTTAGTAGTAAACAAAGAATATTCACAAAATTATAACTGTATCCTTGCGACTCTAAACA
AGCGTTGTTGGTTGTTACAAAACGACCTGGAATTATGGGATATTTTGTGCTGTACCCATC
TTACCCCACGATGCTATAAATTCTGATTAAAAAGCGACAAGTTTGTCAATAGTAATTTGG
AGCTTATAATTGACGAAACAAAGTAATGGTTCGGTTTATAAATGAAAATAATTGAAATAT
ATACTGGAACATTCTTTATCGATTAGGAGAATGACTTTTGAAAAATATAAATAATTTCTT
TATAAAATCGGGTTGGAAAATAGAAATAAAATAAGACAGTAATGTGCCGGTATACATTTT
TTCTATATTGATTAAAATTGAAACGTAAAATAAAATTGTCAAAACGCAAAGTGGAATACA
GCGTAAGACAATAAATGTAATTGGACAGTTACGGCTTAGTTTAATTGCCATAATTTTGTT
TACTACCAAATGGAACGGTAAAGTAGAATAAAGTCCTGGACCATCTTACCCAACCTATAC
TGTATTTAAAGACGACGTAAATTGGCATGTAGTCGCATTTAAATTCTGAGCAATATTTTG
TGTCTCTCGGCAGCGCGCATGAGGTTCGGCGTATAAG

Reference(s)

Stolfi et al. (2015). Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527(7578):371-4

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