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CIS-REGULATION

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  2. Region 'REG00000035' > 
  3. Region 'REG00001590' > 
  4. Transcript 'KH2012:KH.S390.6.v2....' > 
  5. Region 'REG00001593'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S544:4637-5236_FoxDmut|Dmrt1

Short name

Dmrt1_486+µFoxd (Tokuhiro et al., 2017)

Region ID

REG00001593

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Shin-ichi Tokuhiro ()

Annotator

Lea Girard (2023-03-10)

Curator

Marion Gueroult-Bellone (2023-03-21)

View position in WashU

Description

When compared to the unmutated -486bp cis-regulatory element, this 486+µFoxd element has a similar activity in anterior neural a6.5 cells but an increased activity in vegetal cells.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.Kh2012.S544:4637-5236|Dmrt1 by substitution

Regulated gene(s)

KH2012:KH.S544.3 (DMRT1; DMRTA1; DMRTA2)

Construct(s) made to test this region

CONS00001682: p.Cirobu.REG.KH2012.S544:4637-5236_FoxDmut>LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,6375,236

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,637 / 5,236] on scaffold KhS544

There are two TSS in this Dmrt1 cis-regulatory element. The putative FoxD binding site was mutated : TGTTTAC replaced by gtgggca (lowercase in the above sequence). The sequence is 600bp long from the first TSS and 486bp long from the second TSS. This cis-regulatory element is active in anterior neural a6.5 and vegetal cells.

Show sequence

Length: 600

TGTTTTACAGAATCTCAATTTAAAACCCGTCGACAATTATCGGATTTCCGCCGCAGAGTC
ACAGGAAGTTGTTTTGCTTAATAATACTTGTTTTAGATAAGCTGTTATGTTGCTCAATGA
ATAACAATAAgtgggcaGTTGGTTGTTGTGGCAATGTACATTGTAACTTCCACAAACCTC
TCAGAGGGCAAACACATTAAGCAAACTGAATTTGCCAAGTACTTCGGGAAGTCACTGATA
AGCGGACAATACGAAGCGATCACAGGGAATTGTCGCACGCCAGAATGTAAACAAAAACAT
CGCCTGTGCACCGCGCGATCGAAGACACACAGCGCTGACTAATTGACCAACACAAAAGCG
AGGAAAGAAAAAAAAGCGAAAACGAGAACGGGATATTAATTGTACACGATTGTGACGTAA
ATTCGAGGCATTTTTTCTTCCGAAATCGAGCGGTTTAAATACCTCGCGTCTTTATTAGGT
ACAATCATATTGCCTTGATACCTTGCTTAGAACAGTGAATACATATAAATCGTATTAAAA
GCTTTAGAGTGTTAAACCAAAGAGTTACAGAAATTATAGCAAACAGTTCGTTTAACTGGC

Reference(s)

Tokuhiro et al. (2017). Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd. PLoS Genet. 13(5):e1006741

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