Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00001591'

Cis-regulatory Region

Name

Cirobu.REG.Kh2012.S544:4837-5236|Dmrt1

Short name

Dmrt1_-286bp (Tokuhiro et al., 2017)

Region ID

REG00001591

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Shin-ichi Tokuhiro (2023-03-10)

Annotator

Lea Girard (2023-03-10)

Curator

Marion Gueroult-Bellone (2023-03-21)

View position in WashU

Description

When compared to the -343bp cis-regulatory region, the -286bp element has a decreased activity in anterior neural a6.5 cells but a similar activity in vegetal cells.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.Kh2012.S544:4780-5236|Dmrt1 by deletion

Regulated gene(s)

KH2012:KH.S544.3 (DMRT1; DMRTA1; DMRTA2)

Construct(s) made to test this region

CONS00001680: p.Cirobu.REG.Kh2012.S544:4837-5236>LacZ

Show in situ data
Overview of functional TF binding sites in this region

4,8375,236

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [4,837 / 5,236] on scaffold KhS544

There are two TSSs in this Dmrt1 cis-regulatory element. The sequence is 400bp long from the first TSS and 286bp long from the second TSS of Dmrt1. This cis-regulatory element is active in anterior neural cells a6.5 and in vegetal cells.

Show sequence

Length: 400

GCAAACTGAATTTGCCAAGTACTTCGGGAAGTCACTGATAAGCGGACAATACGAAGCGAT
CACAGGGAATTGTCGCACGCCAGAATGTAAACAAAAACATCGCCTGTGCACCGCGCGATC
GAAGACACACAGCGCTGACTAATTGACCAACACAAAAGCGAGGAAAGAAAAAAAAGCGAA
AACGAGAACGGGATATTAATTGTACACGATTGTGACGTAAATTCGAGGCATTTTTTCTTC
CGAAATCGAGCGGTTTAAATACCTCGCGTCTTTATTAGGTACAATCATATTGCCTTGATA
CCTTGCTTAGAACAGTGAATACATATAAATCGTATTAAAAGCTTTAGAGTGTTAAACCAA
AGAGTTACAGAAATTATAGCAAACAGTTCGTTTAACTGGC

Reference(s)

Tokuhiro et al. (2017). Differential gene expression along the animal-vegetal axis in the ascidian embryo is maintained by a dual functional protein Foxd. PLoS Genet. 13(5):e1006741

Back to Top