- Region 'REG00001574'
Cis-regulatory Region
Name
Cirobu.REG.KhL96:469627-471527|Tbx2/3
Short name
Tbx2/3 intron #3 (notochord 1937bp CRM) cluster (José-Edwards 2013)
Region ID
REG00001574
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(enhancer)
Author
Diana S. José-Edwards (2023-02-23)
Annotator
Marion Gueroult-Bellone (2023-02-23)
Curator
Marion Gueroult-Bellone (2023-02-23)
- Cirobu.REG.KhL96:469627-471527|Tbx2/3 REG00001574 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527|Tbx2/3 REG00001575 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox5-6-7-mut|Tbx2/3 REG00001585 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox5-7-mut|Tbx2/3 REG00001584 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox6-7-mut|Tbx2/3 REG00001583 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox5-6-mut|Tbx2/3 REG00001582 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox7-mut|Tbx2/3 REG00001581 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox6-mut|Tbx2/3 REG00001580 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527_Tbox5-mut|Tbx2/3 REG00001579 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471379-471527|Tbx2/3 REG00001577 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471407|Tbx2/3 REG00001576 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhL96:471236-471527|Tbx2/3 REG00001575 [ regulatory_region (enhancer) ]
469,627471,527
Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
---|
Aniseed Coordinates: [469,627 / 471,527] on scaffold KhL96
This 2917bp region is located in the 3rd and 2nd introns of Tbx2/3. Primers used to amplify this region : Tbx2/3 intron #3 cluster F: TtctagaCATGGTGCTTTGGACGCGTTACC XbaI and Tbx2/3 intron #3 cluster R: ATccatggGGAATGGGGATTACGGTAGTCGAC This region is active mainly in the notochord, and a bit in tail muscles and epidermis. Note that sequence in the article is 1937bp long, and only 1901bp long when blasting primers against KH sequence assembly.
CATGGTGCTTTGGACGCGTTACCAGACATGCCTCCCTTGATATATCCATGCTGAGTTTTA
CAGCGTTATATTCAGTGCCATAACCCGAGTTTGAAAGGGACATAATTCGTCAAAGCGTTC
TTGTGGCCGGTTAGGCCCATACTAAGGTCAGCTTGGCGCCATGAACAGTTAGCAGCCAGC
AGGGGTCCATAGGGAGAAAGAGGCTTAACGTACGGTTCTGTGTTAGACGCCTTAGAGATG
CCGGCGTCTCAGCACGTGTTAGAAGATATTTATTAACAAATTTGATTTACATCAATGGCA
CGGTTAATTGTCTGCTTCTCACAGATTGTGCAGTTAACCCCGAACGTAGTTTATTAACCT
AATGGAAAGTTCGCTCTCTCGGGCTTTGGAGCGTCGGCTCTATTTTAACTCCTAGCTTTT
ATTTGCAGACCATTTTGAACTCAATGCACAAGTACCAGCCACGCTTCCACGTTGTGAGGT
GTGGGGACCTTGCGAAGCTTCCATACTGTGCGTTCAGGACGTACGTGTTCAAGGAGATGC
AGTTCATCGCAGTGACAGCATACCAGAACGAGAAGGTGACCGTCAAGTTACTTATTACGC
CTACCGTGCGATTTTAAGTCGTTTTGCCTCCTTATCAACAGTCAGAACAATTCTGGCCGC
CGTGGTATCTTAACACTGGCGACTCTAACTCTGTAGGAGTCACGACTGCCACTTACAAAG
GCGCCTATGTTTGATCCCACCCGATAGTGAGATGTTATGTAAGCATCTTTAGAATCCCAG
CTATTACGTTCGTGTGGGAAGAAGTCCTACTGTCCAAGACGTGTTTATACGCCAGGCGTC
ATGTGCTTGAAACTTACAAGTAGAGAGTTAACGCTTTCACTGCGAATACGACACACGTCT
TTGTCAACTTAGGAAACATTCTATGAATTGTTTGAAGTGCTAGGGTGGCGGCGACGTTTA
CACTCTTTTAGATCAAAGCAACGTCGTGTTAGCGCCTTGAGCCTTATGATCTCATAATGG
CTATGAAAACATAGATATCAGATACTAAGTTTAATAAGCGGGGAGTCGAAACCGAAATCG
GAAAATCTTGTTACATTCGCTTGTATTGAGACTTTTGAAGTACCGGCGAGTGGGCATGGC
TCATTTTTGAAATTCTTTTTTTTTGCGTTGGAAATGAAACTGAAACAAACTACACGCTCG
AGGTGGCTCAAAACCATTACAAAAGCTGTGCTAGCACGTCGCCTGCGCTTTGTAGACAGC
CAGCATACTTGACCAAGGGTGCGATTGCGAATCAAAACAAACAGATAAGATGGTAGGAGC
GGTCGTTCAATTTGTTTTAGCCTTCATTCTCCTCTGTGAGTTGACAATTGAACGACACGC
ACTTCTCGGGAACTTAGCTTTAATGTACAAAGTCCCACACCTGGCGCAATACATGTGTTA
TGACACGCTCTATCGCCTAGTTTCTGAAATCTCGCCAGACAAGACGTTGCAATTTCCCCG
GCCAGAAAACATGGACAATGTGTTCAAACACAAACATTTCCTGTAAACCACTCAAAAGGC
CCTTAAAATGACGCCTGAAATAGGCATAGGAATTTAAACGTTATAATGTGTGGACAGACA
GGGTAGTTAAATACGTTACAAAGTTCATTTGCCACCCAGCACAGTATTGTACTTTATGAC
ACTTGTGTTTATTCGTATAGTGGGACTAGTTATAAAATAAAACGGTTTATTCTCTGACAC
GTAAGTGTTAATCTATAAGCAATGTGACAAGAACAAATAACACGAAGGGGCGAAATCTAA
CGCTAGATCAACTGCGTGGCTTTCACATCTTTGGTGGTCGAGTAATACATAGCACTAAAC
CGAAACAATTATTTTTCGTCGACTACCGTAATCCCCATTCC