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CIS-REGULATION

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  1. Region 'REG00001569'

Cis-regulatory Region

Name

Cirobu.REG.Kh2012.C1:823000-823167|Nkxtun3

Short name

Cirobu.Nkxtun3-up3

Region ID

REG00001569

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Joshua F Coulcher (2023-02-22)

Annotator

Marion Gueroult-Bellone (2023-02-22)

Curator

Marion Gueroult-Bellone (2023-02-22)

View position in WashU

Description

Compared to its mother sequence, it lacks 110bp in 3'. It's weak activity in dorsal and ventral neurogenic midlines is abrogated by the deletion.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.Kh2012.C1:823000-823274|Nkxtun3 by deletion

Regulated gene(s)

KH2012:KH.C1.922 (BARHL1; BARHL2; TLX1)

Construct(s) made to test this region
n/a
Overview of functional TF binding sites in this region

823,000823,166

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [823,000 / 823,166] on scaffold KhC1

This 168bp regulatory region is located upstream of Nkxtun3 (Nkxtun3-Up3) and is not active in dorsal and ventral neurogenic midlines. Genome coordinates are in the article.

Show sequence

Length: 167

CAGCGTGTCCGTTTGTTTCCTTGTTAATAGAACTTAATTACAGGGTGTCTGTTGCTATGA
TGACAAGATAAGATGGCGGGCGCTTCTCATCTGGCTTGTTTGGCAACAATTGATCGATAG
CCAAATCGCATTATGAGTCATAACTAAAATTCGAATGCGGGTGGTGA

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():

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