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CIS-REGULATION

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  1. Region 'REG00001568'

Cis-regulatory Region

Name

Cirobu.REG.Kh2012.C1:823000-823274|Nkxtun3

Short name

Cirobu.Nkxtun3-up8

Region ID

REG00001568

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Joshua F Coulcher (2023-02-22)

Annotator

Marion Gueroult-Bellone (2023-02-22)

Curator

Marion Gueroult-Bellone (2023-02-22)

View position in WashU

Description

Compared to its mother sequence, it lacks 50bp in 3'. This loss drastically reduced the enhancer activity in dorsal and ventral neurogenic midlines.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.Kh2012.C1:823000-823325|Nkxtun3 by deletion

Regulated gene(s)

KH2012:KH.C1.922 (BARHL1; BARHL2; TLX1)

Construct(s) made to test this region
n/a
Overview of functional TF binding sites in this region

823,000823,273

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [823,000 / 823,273] on scaffold KhC1

This 275bp regulatory region is located upstream of Nkxtun3 (Nkxtun3-Up8) and is very weakly active in dorsal and ventral neurogenic midlines. Genome coordinates are in the article.

Show sequence

Length: 274

CAGCGTGTCCGTTTGTTTCCTTGTTAATAGAACTTAATTACAGGGTGTCTGTTGCTATGA
TGACAAGATAAGATGGCGGGCGCTTCTCATCTGGCTTGTTTGGCAACAATTGATCGATAG
CCAAATCGCATTATGAGTCATAACTAAAATTCGAATGCGGGTGGTGAATTCGAACAAAAA
CGTCGCGCTAACTCGACAGCACTATTGCGTCATAAAAAGTCGTAAAGGCGCGCGCTTAAA
CGAGTAATTACGGAAAAATTATGACATCGTTTAA

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():

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