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CIS-REGULATION

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  1. Region 'REG00001567'

Cis-regulatory Region

Name

Cirobu.REG.Kh2012.C1:823000-823325|Nkxtun3

Short name

Cirobu.Nkxtun3-up7 (Coulcher 2020)

Region ID

REG00001567

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Joshua F Coulcher (2023-02-22)

Annotator

Marion Gueroult-Bellone (2023-02-22)

Curator

Marion Gueroult-Bellone (2023-02-22)

View position in WashU

Description

Compared to its mother sequence, it lacks 70bp in 3' side. It is active in more embryos (>60%).

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C1.922 (BARHL1; BARHL2; TLX1)

Construct(s) made to test this region

CONS00001662: p.Cirobu.REG.Kh2012.C1:823000-823325>LacZ

Show in situ data
Overview of functional TF binding sites in this region

823,000823,324

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [823,000 / 823,324] on scaffold KhC1

This 326bp regulatory region is located upstream of Nkxtun3 (Nkxtun3-Up7) and is equaly active in both dorsal and ventral neurogenic midlines. Genome coordinates are in the article.

Show sequence

Length: 325

CAGCGTGTCCGTTTGTTTCCTTGTTAATAGAACTTAATTACAGGGTGTCTGTTGCTATGA
TGACAAGATAAGATGGCGGGCGCTTCTCATCTGGCTTGTTTGGCAACAATTGATCGATAG
CCAAATCGCATTATGAGTCATAACTAAAATTCGAATGCGGGTGGTGAATTCGAACAAAAA
CGTCGCGCTAACTCGACAGCACTATTGCGTCATAAAAAGTCGTAAAGGCGCGCGCTTAAA
CGAGTAATTACGGAAAAATTATGACATCGTTTAAATTTCCTACAACTGTTTACGTCGTAA
GCGTCTCATTCATTGTGGGCGTTTA

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():

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