Login
Help

CIS-REGULATION

Submit your Data

  1. Region 'REG00001566'

Cis-regulatory Region

Name

Cirobu.REG.Kh2012.C1:823069-823390|Nkxtun3

Short name

Cirobu.Nkxtun3-up5 (Coulcher 2020)

Region ID

REG00001566

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Joshua F Coulcher (2023-02-22)

Annotator

Marion Gueroult-Bellone (2023-02-22)

Curator

Marion Gueroult-Bellone (2023-02-22)

View position in WashU

Description

Compared to its mother sequence (Nkxtun3-Up1), it lacks 70bp from 5'. Its activity in ventral neurogenic midline is maintained, but was 2-fold reduced in dorsal neurogenic midline.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C1.922 (BARHL1; BARHL2; TLX1)

Construct(s) made to test this region

CONS00001661: p.Cirobu.REG.Kh2012.C1:823069-823390>LacZ

Show in situ data
Overview of functional TF binding sites in this region

823,069823,389

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [823,069 / 823,389] on scaffold KhC1

This 322bp regulatory region is located upstream of Nkxtun3 (Nkxtun3-Up5) and is strongly active in dorsal and ventral neurogenic midlines. Genome coordinates are in the article.

Show sequence

Length: 321

AAGATGGCGGGCGCTTCTCATCTGGCTTGTTTGGCAACAATTGATCGATAGCCAAATCGC
ATTATGAGTCATAACTAAAATTCGAATGCGGGTGGTGAATTCGAACAAAAACGTCGCGCT
AACTCGACAGCACTATTGCGTCATAAAAAGTCGTAAAGGCGCGCGCTTAAACGAGTAATT
ACGGAAAAATTATGACATCGTTTAAATTTCCTACAACTGTTTACGTCGTAAGCGTCTCAT
TCATTGTGGGCGTTTAACAAAGTAAAGACCGAAAGGTGACAATTATCCAGTAAAACCCAG
CCCAGTCAAAATCATCAGGGC

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():

Back to Top