Description

Compared to its mother sequence (Nkxtun3-Upstream), it lacks the TSS-proximal region and the 5' region. It covers the most conserved region between Ciona robusta and Ciona savignyi.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C1.922 (BARHL1; BARHL2; TLX1)

Construct(s) made to test this region

CONS00001660: p.Cirobu.REG.Kh2012.C1:823000-823390>LacZ

Overview of functional TF binding sites in this region

823,000823,389

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [823,000 / 823,389] on scaffold KhC1

This 391bp cis-regulatory region is located upstream of Nkxtun3 (Nkxtun3-up1) and is active in dorsal and ventral neurogenic midlines (40% of the embryos). Genome coordinates are in the article.

Reference(s)

Coulcher et al. (2020). Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos. Elife 9():