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CIS-REGULATION

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  1. Clone 'cien114535' > 
  2. Clone 'cien214335' > 
  3. Region 'REG00001556' > 
  4. Region 'REG00001560' > 
  5. Region 'REG00001562'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L59:1807-2057_FoxMut|Zic-r.b

Short name

Zic-r.b -251bp FoxMut (Imai 2016)

Region ID

REG00001562

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Kaoru S. Imai (2023-01-17)

Annotator

Marion Gueroult-Bellone (2023-01-17)

Curator

Marion Gueroult-Bellone (2023-01-17)

View position in WashU

Description

Mutation of the 4 proximal Fox binding sites of the 251bp cis-regulatory region of Zic-r.b (KhL59.1) strongly affects is activity : NN (A6.2 and A6.4 lineages) activity is lost, aMM (B6.2 lineage) activity is strongly reduced and pMM (B6.4) is maintained

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L59:1807-2057|Zic-r.b by substitution

Regulated gene(s)

KH2012:KH.L59.1 (ZIC2; ZIC3; ZIC4)

Construct(s) made to test this region

CONS00001645: p.Cirobu.REG.KH2012.L59:1807-2057_FoxMut>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,8072,056

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,807 / 2,056] on scaffold KhL59

Four putative Fox binding sites were mutated (lower case letters).

Show sequence

Length: 250

TTTCGTAACACAATGACTTAATTATCTTCTTTATCTTTAgggcTTTGACTTCCGATCGCC
CGTCGCTTTCCCATGCGCACGTGGTTCGACCCCCCACCTCCGAtcggTACATCGCGAAGG
TAATTATCGTGTCGATCGCTCGTCTCGCGGTACTTTTGGCACGCTTTCGCTCGTGCCTGT
CTACGCAAGGTTTACCTATGGCACTTTCTCTACGTGAAGTCAcgcACAAGTgcgACATTG
CAGAGTGGTT

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

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