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CIS-REGULATION

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  1. Region 'REG00001558' > 
  2. Region 'REG00001557'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L59:1807-2086_GATAmut1|Zic-r.b

Short name

Zic-r.b -280bp GATAmut(1) (Imai 2016)

Region ID

REG00001557

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Kaoru S. Imai (2022-11-28)

Annotator

Marion Gueroult-Bellone (2022-11-28)

Curator

Marion Gueroult-Bellone (2022-11-28)

View position in WashU

Description

Mutation of the 2 proximal GATA binding sites strongly reduced the enhancer activity in all the marginal cells.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L59:1807-2086|Zic-r.b by substitution

Regulated gene(s)

KH2012:KH.L59.1 (ZIC2; ZIC3; ZIC4)

Construct(s) made to test this region

CONS00001641: p.Cirobu.REG.KH2012.L59:1807-2086_GATAmut1>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,8072,085

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,807 / 2,085] on scaffold KhL59

The 2 proximal GATA binding sites were mutated : GATA replaced by aATA (lowercase in the above sequence).

Show sequence

Length: 279

TTTCGTAACACAATGACTTAATTATtTTCTTTATtTTTATTTATTTGACTTCCGATCGCC
CGTCGCTTTCCCATGCGCACGTGGTTCGACCCCCCACCTCCGAAATTTACATCGCGAAGG
TAATTATCGTGTCGATCGCTCGTCTCGCGGTACTTTTGGCACGCTTTCGCTCGTGCCTGT
CTACGCAAGGTTTACCTATGGCACTTTCTCTACGTGAAGTCAACAACAAGTTACACATTG
CAGAGTGGTTTATGCAAAAAAGACCAAAAAACAGGACGT

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

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