Login
Help

CIS-REGULATION

Submit your Data

  1. Clone 'cien214335' > 
  2. Region 'REG00001556' > 
  3. Region 'REG00001560' > 
  4. Region 'REG00001562' > 
  5. Region 'REG00001555'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L59:1807-2488_GATAmut|Zic-r.b

Short name

Zic-r.b -682bp GATAmut (Imai 2016)

Region ID

REG00001555

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Kaoru S. Imai (2022-11-28)

Annotator

Marion Gueroult-Bellone (2022-11-28)

Curator

Marion Gueroult-Bellone (2022-11-28)

View position in WashU

Description

Mutation of the 2 proximal GATA binding site of the 681bp cis-regulatory region of Zic-r.b (KhL59.1) greatly reduced its enhancer activity in both NN (notochord and nerve chord, A6.2 and A6.4) and MM lineages (Muscle, mesenchyme and notochord, B6.2 and B6.4).

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L59:1807-2488|Zic-r.b by substitution

Regulated gene(s)

KH2012:KH.L59.1 (ZIC2; ZIC3; ZIC4)

Construct(s) made to test this region

CONS00001639: p.Cirobu.REG.KH2012.L59:1807-2488_GATAmut>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,8072,487

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,807 / 2,487] on scaffold KhL59

Two proximal Gata sites were mutated : AGATAAAGAAGATAA replaced by AGcgAAAGAAGcgAA (lowercase in the above sequence).

Show sequence

Length: 681

TTTCGTAACACAATGACTTAATTcgCTTCTTTcgCTTTATTTATTTGACTTCCGATCGCC
CGTCGCTTTCCCATGCGCACGTGGTTCGACCCCCCACCTCCGAAATTTACATCGCGAAGG
TAATTATCGTGTCGATCGCTCGTCTCGCGGTACTTTTGGCACGCTTTCGCTCGTGCCTGT
CTACGCAAGGTTTACCTATGGCACTTTCTCTACGTGAAGTCAACAACAAGTTACACATTG
CAGAGTGGTTTATGCAAAAAAGACCAAAAAACAGGACGTTCATCGATATTTAAAAAAAAT
ATATAATAAATTATAATTGTTAATAATTTTTACGTATTCGATTTTTAATTATTTTGTTTA
ATAAAAAATATTGCATGTATTTGCCGGCTTATTTTTTTATGTTTGATTTAACTACTAATA
TTAGCATACCGATTGTGTTAAAATTATAGTTTATCAGATTTTTTTTATATGCATGTTCTT
AAACTTTTTAAAATTATAAAATAATCTTATAACAACCTAGATAACAGCAAGTCATTGCGA
ATTCAACATTCCAGACCCTTTGTTAATTGTTACGTCATCATTGTTTAAAATTCGGGTTGT
CTCGAGCCATTGTAGCGTGTGTGAAGATTATAGAGGATTATAATTTAAATATCATTTAGT
GAACGGAAATGACATAAACTA

Reference(s)

Imai et al. (2016). Antagonism between β-catenin and Gata.a sequentially segregates the germ layers of ascidian embryos. Development 143(22):4167-4172

Back to Top