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CIS-REGULATION

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  1. Region 'REG00001548'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333930-1333979|Rx

Short name

J-E_Rx-502/-453

Region ID

REG00001548

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

D Aniello Enrico (2022-10-13)

Annotator

Marion Gueroult-Bellone (2022-10-13)

Curator

Marion Gueroult-Bellone (2022-10-13)

View position in WashU

Description

This 50bp element is located upstream (-502/-453bp) of Rx TSS. Associated with the basal promoter of the human β-globin, it is not able to drive reporter gene activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333829-1334025|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001622: p.Cirobu.REG.KH2012.C12:1333930-1333979>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,9301,333,979

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,930 / 1,333,979] on scaffold KhC12

This 50bp DNA fragment was amplified using the following annealing oligonucleotides : J-E up: AACGAGTAATTTAACATAATTAATAAAAGAGACACAGCAGTGCATTTTCA and J-E down: TGAAAATGCACTGCTGTGTCTCTTTTATTAATTATGTTAAATTACTCGTT

Show sequence

Length: 50

AACGAGTAATTTAACATAATTAATAGAAGAGACATAGCAGTGCATTTTCA

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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