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CIS-REGULATION

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  1. Clone 'cima822650' > 
  2. Region 'REG00000915' > 
  3. Region 'REG00001547'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333901-1333934|Rx

Short name

J-D_Rx-531/-498

Region ID

REG00001547

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

D Aniello Enrico (2022-10-11)

Annotator

Marion Gueroult-Bellone (2022-10-11)

Curator

Marion Gueroult-Bellone (2022-10-11)

View position in WashU

Description

This 34bp element is located upstream (-531/-498bp) of Rx TSS. Associated with the basal promoter of the human β-globin, it is not able to drive reporter gene activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333829-1334025|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001621: p.Cirobu.REG.KH2012.C12:1333901-1333934>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,9011,333,934

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,901 / 1,333,934] on scaffold KhC12

This 34bp DNA fragment was amplified using the following annealing oligonucleotides : J-D up: TAATCCTTATTAAAATGTCGAAGTATTATAACGA and J-D down: TCGTTATAATACTTCGACATTTTAATAAGGATTA

Show sequence

Length: 34

TAATCCTTATTAAAATGTCGAAGTATTATAACGA

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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