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CIS-REGULATION

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  1. Region 'REG00001542'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1334069-1334401|Rx

Short name

L0.33_Rx-363/-31

Region ID

REG00001542

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(RNApol_II_core_promoter)

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

This 333bp element is located uptream (-363/-31) of Rx TSS. Cloned in a reporter construct, it can only drive ectopic LacZ expression in the mesenchyme.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333915-1334401|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001616: p.Cirobu.REG.KH2012.C12:1334069-1334401>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,334,0691,334,401

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,334,069 / 1,334,401] on scaffold KhC12

This 333bp DNA fragment was amplified using the following primers : L 0.33 up: CACGTCGTAATTCGTACTAAAC and L 0.33 down: CTAAATAACGAAATATGAATTAAAATC

Show sequence

Length: 333

CACGTCGTAATTCGTACTAAACAGATTGCTCTTTGTGATTTTCAATCACTTCCAAATCTT
TGCTTACTCTTTAAACTAGGAGCGAAGAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGT
TAAAACACAGTGCTTATGACGACATAGCTGTAATACTGAAGTGATTGTTCGGTAAGAAAG
AAGTGAACTCAAAGCGGACATTTTACAGTAAGGCTTAGAAAGTCAAGTACAGTATATTTA
ATAATGCATTCTAAAGCATATTTCATGGACACTTCATTCATTTTATTATTATAAAAAAAT
TAATTAGATTTTAATTCATATTTCGTTATTTAG

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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