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CIS-REGULATION

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  1. Region 'REG00001541'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333915-1334188|Rx

Short name

K0.27_Rx-517/-244

Region ID

REG00001541

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

This 274bp enhancer is located upstream (-517/-244) of Rx TSS. Associated with the basal promoter of the human β-globin, it drives reporter gene activity in the central nervous system and ectopic mesenchyme of Ciona robusta tailbuds and larvae.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333915-1334401|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001615: p.Cirobu.REG.KH2012.C12:1333915-1334188>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,9151,334,188

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,915 / 1,334,188] on scaffold KhC12

This 274bp enhancer was amplified using the following primers : K 0.27 up: ATGTCGAAGTATTATAACGAG and K 0.27 down: ACCAGGTTCTGGAACAAATTGC

Show sequence

Length: 274

ATGTCGAAGTATTATAACGAGTAATTTAACATAATTAATAGAAGAGACATAGCAGTGCAT
TTTCAAGTAGAATCAATACTAGCATTACCATCTGGCGATACACGGCCATATATACTCGGC
CCGGCTGCTATAATTTTTCAATAATTCCTGCTCACACGTCGTAATTCGTACTAAACAGAT
TGCTCTTTGTGATTTTCAATCACTTCCAAATCTTTGCTTACTCTTTAAACTAGGAGCGAA
GAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGT

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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