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CIS-REGULATION

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  1. Region 'REG00001540'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333829-1334025_OnecutMUT|Rx

Short name

J0.2-MUT_Rx-603/-407

Region ID

REG00001540

Status

Curated

Origin

engineered region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

The point mutations in five nucleotides of the core Onecut binding sequence decreased the enhancer efficiency of the WT J0.2 construct.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333829-1334025|Rx by substitution

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001614: p.Cirobu.REG.KH2012.C12:1333829-1334025_OnecutMUT>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,8291,334,025

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,829 / 1,334,025] on scaffold KhC12

The conserved putative Onecut binding site was mutated : AAGTAGAATCAATACTA was replaced by AAGTAGAcTCccACcA (small letters in the sequence). The construct was prepared by site-directed mutagenesis from the J0.2 construct with Quik Change Site-Directed Mutagenesis Kit (Stratagene).

Show sequence

Length: 197

ATCGCGTTTTCATTTTATCAATAATACCCTTAAGGGTCAGCAAGTATGAAACGCAATTAG
TGCAAATTGCCCTAATCCTTATTAAAATGTCGAAGTATTATAACGAGTAATTTAACATAA
TTAATAGAAGAGACATAGCAGTGCATTTTCAAGTAGAcTCcccACcAGCATTACCATCTG
GCGATACACGGCCATAT

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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