Login
Help

CIS-REGULATION

Submit your Data

  1. Transcript 'KH2012:KH.C2.718.v1....' > 
  2. Transcript 'Haaura.CG.MTP2014.S2...' > 
  3. Transcript 'KH2012:KH.S999.1.v1....' > 
  4. Transcript 'KH2012:KH.C8.713.v1....' > 
  5. Region 'REG00001536'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333915-1334401|Rx

Short name

F0.49_Rx-517/-31

Region ID

REG00001536

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

D Aniello Enrico (2022-10-10)

Annotator

Marion Gueroult-Bellone (2022-10-10)

Curator

Marion Gueroult-Bellone (2022-10-10)

View position in WashU

Description

This 487bp cis-regulatory element is located upstream (-517/-31) of Rx TSS. It drives reporter gene activity in the central nervous system of Ciona robusta tailbuds and larvae, and ectopically in the mesenchyme.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333114-1334401|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001610: p.Cirobu.REG.KH2012.C12:1333915-1334401>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,9151,334,401

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,915 / 1,334,401] on scaffold KhC12

This 487bp DNA fragment was amplified using the following primers : F 0.49 up: ATGTCGAAGTATTATAACGAG and F 0.49 down: CTAAATAACGAAATATGAATTAAAATC

Show sequence

Length: 487

ATGTCGAAGTATTATAACGAGTAATTTAACATAATTAATAGAAGAGACATAGCAGTGCAT
TTTCAAGTAGAATCAATACTAGCATTACCATCTGGCGATACACGGCCATATATACTCGGC
CCGGCTGCTATAATTTTTCAATAATTCCTGCTCACACGTCGTAATTCGTACTAAACAGAT
TGCTCTTTGTGATTTTCAATCACTTCCAAATCTTTGCTTACTCTTTAAACTAGGAGCGAA
GAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGTTAAAACACAGTGCTTATGACGACATA
GCTGTAATACTGAAGTGATTGTTCGGTAAGAAAGAAGTGAACTCAAAGCGGACATTTTAC
AGTAAGGCTTAGAAAGTCAAGTACAGTATATTTAATAATGCATTCTAAAGCATATTTCAT
GGACACTTCATTCATTTTATTATTATAAAAAAATTAATTAGATTTTAATTCATATTTCGT
TATTTAG

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

Back to Top