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CIS-REGULATION

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  1. Region 'REG00001534'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333114-1333594|Rx

Short name

D0.48_Rx-1318/-838

Region ID

REG00001534

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

D Aniello Enrico (2022-10-06)

Annotator

Marion Gueroult-Bellone (2022-10-06)

Curator

Marion Gueroult-Bellone (2022-10-06)

View position in WashU

Description

This 481bp element is located upstream (-1318/-838) of Rx TSS. When cloned upstream the human β-globin basal promoter and LacZ reporter gene, it has no enhancer activity.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.C12:1333114-1334401|Rx by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001608: p.Cirobu.REG.KH2012.C12:1333114-1333594>LacZ

Show in situ data
Overview of functional TF binding sites in this region

1,333,1141,333,594

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,114 / 1,333,594] on scaffold KhC12

This 481bp genomic sequence was amplified using the following primers : D 0.48 up: CACAATGGAACCATGGAAAG and D 0.48 down: GACAGTAACTTAGTGGATAGCAACC

Show sequence

Length: 481

CACAATGGAACCATGGAAAGATCTTTTGTCCTATATACTGAAGAATATACGATTTCGAGT
CACGACGCTGCTACACCATTGTGGGCGTATATGTGTTTTTAAAAATAATTTATTTTCGGA
AATTGCTTCAACGTATTGTTAGATTATGGGTTGTCTAAAGTTTTAGTCCCACATTAAAAA
ACAATTCAAAAGTAATTCGTAAGCAAGCACGATGTAATTAAGGCTAACCGCGTTTTTTAC
GCCATGCGAGGATATACAAGTTACATTTATTCAATAACACTGCATCGTTTCTAATAACAA
TGATGGGTTACATTACAATAACATATATATACGTACTTATAGTCAAGACAAACTATAGTA
GCCGTGTTTTAGTCTATTACCCTACTGCACGAAACATCTTTTGAATATCATTTTTAGCAT
CCAGACAGTAACTTGGTCCATCACCAGTGTAAAAAAGGTTGCTATCCACTAAGTTACTGT
C

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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