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CIS-REGULATION

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  1. Region 'REG00000142' > 
  2. Transcript 'Phmamm.CG.MTP2014.S4...' > 
  3. Region 'REG00001223' > 
  4. Region 'REG00001533'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.C12:1333114-1334401|Rx

Short name

C1.3_Rx-1318/-31

Region ID

REG00001533

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(complex_region)

Author

D Aniello Enrico (2022-10-06)

Annotator

Marion Gueroult-Bellone (2022-10-06)

Curator

Marion Gueroult-Bellone (2022-10-06)

View position in WashU

Description

This 1288bp regulatory element is located upstream (-1318/-31) of Rx (KH.C12.152) TSS. When cloned upstream of GFP or LacZ it drives reporter gene expression in the central nervous system at tailbud and larval stages.

Hierarchy of Regulatory Regions

Modification of Ci-Rx -3kb/1 by deletion

Regulated gene(s)

KH2012:KH.C12.152 (PAX6; RAX; RAX2)

Construct(s) made to test this region

CONS00001607: p.Cirobu.REG.KH2012.C12:1333114-1334401>LacZ

CONS00001606: p.Cirobu.REG.KH2012.C12:1333114-1334401>GFP

Show in situ data
Overview of functional TF binding sites in this region

1,333,1141,334,401

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [1,333,114 / 1,334,401] on scaffold KhC12

This 1288bp cis-regulatory sequence was amplified using the following primers : C 1.3 up: CACAATGGAACCATGGAAAG and C 1.3 down: CTAAATAACGAAATATGAATTAAAATC

Show sequence

Length: 1,288

CACAATGGAACCATGGAAAGATCTTTTGTCCTATATACTGAAGAATATACGATTTCGAGT
CACGACGCTGCTACACCATTGTGGGCGTATATGTGTTTTTAAAAATAATTTATTTTCGGA
AATTGCTTCAACGTATTGTTAGATTATGGGTTGTCTAAAGTTTTAGTCCCACATTAAAAA
ACAATTCAAAAGTAATTCGTAAGCAAGCACGATGTAATTAAGGCTAACCGCGTTTTTTAC
GCCATGCGAGGATATACAAGTTACATTTATTCAATAACACTGCATCGTTTCTAATAACAA
TGATGGGTTACATTACAATAACATATATATACGTACTTATAGTCAAGACAAACTATAGTA
GCCGTGTTTTAGTCTATTACCCTACTGCACGAAACATCTTTTGAATATCATTTTTAGCAT
CCAGACAGTAACTTGGTCCATCACCAGTGTAAAAAAGGTTGCTATCCACTAAGTTACTGT
CTGAAACATCTGCTGATCATGAGTTTATTATCGACGTATTGTTAAACTAAAACATAGTCA
AACGCAAAGTCTTAATCGTAACACATTTATTTTAGAATATTATAAAAAACGGTACCATAC
TTTACCGATGTTTTTTAAAATTTGTTTGTTTAATTTTGATACTTCCTTCAGCCCATTTGT
TTTTGTGTGTGCTTATGCGGTACAATCTAAATTATAATTATTCCCTTAAGAATCGATCGC
GTTTTCATTTTATCAATAATACCCTTAAGGGTCAGCAAGTATGAAACGCAATTAGTGCAA
ATTGCCCTAATCCTTATTAAAATGTCGAAGTATTATAACGAGTAATTTAACATAATTAAT
AGAAGAGACATAGCAGTGCATTTTCAAGTAGAATCAATACTAGCATTACCATCTGGCGAT
ACACGGCCATATATACTCGGCCCGGCTGCTATAATTTTTCAATAATTCCTGCTCACACGT
CGTAATTCGTACTAAACAGATTGCTCTTTGTGATTTTCAATCACTTCCAAATCTTTGCTT
ACTCTTTAAACTAGGAGCGAAGAAAGGAAAAAGGCAATTTGTTCCAGAACCTGGTTAAAA
CACAGTGCTTATGACGACATAGCTGTAATACTGAAGTGATTGTTCGGTAAGAAAGAAGTG
AACTCAAAGCGGACATTTTACAGTAAGGCTTAGAAAGTCAAGTACAGTATATTTAATAAT
GCATTCTAAAGCATATTTCATGGACACTTCATTCATTTTATTATTATAAAAAAATTAATT
AGATTTTAATTCATATTTCGTTATTTAG

Reference(s)

D'Aniello et al. (2011). Onecut is a direct neural-specific transcriptional activator of Rx in Ciona intestinalis. Dev. Biol. 355(2):358-71

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