- Region 'REG00001523'
Cis-regulatory Region
Name
Cirobu.REG.KhL153:204692-208149|Pitx
Short name
Pitx (P3+P1) (Christiaen 2005)
Region ID
REG00001523
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(enhancer)
Author
Lionel Christiaen (2020-11-21)
Annotator
Marion Gueroult-Bellone (2020-11-21)
Curator
Marion Gueroult-Bellone (2020-11-21)
Unlike the larger fragment, This 3.5kb cis-regulatory element does not drive ectopic expression.
Modification of Ci-Pitx -7865/140 by deletion
- Ci-Pitx -7865/140 REG00000681 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -2735/-2540 REG00000682 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 First Otx site mutated REG00000685 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 Second Otx site mutated REG00000686 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 First Forkhead site mutated REG00000687 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 Second Forkhead site mutated REG00000688 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 First Smad site mutated REG00000689 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 Second, Third, Fourth Smad site mutated REG00000690 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 TALE-class sites mutated REG00000691 [ regulatory_region (enhancer) ]
- Ci-Pitx -2751/-2543 delta -2693/-2646 delta -2606/-2588 REG00000684 [ regulatory_region (complex_region) ]
- Ci-Pitx -2751/-2606 delta -2693/-2646 REG00000692 [ inactive_region ]
- Ci-Pitx -2751/-2696 REG00000693 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2 REG00000694 [ regulatory_region (complex_region) ]
- Ci-Pitx -2751/-2696 repeated x2 Second Pax site deleted REG00000696 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2 Second Otx site mutated REG00000697 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2 Second Forkhead site mutated REG00000698 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2 Second Smad site mutated REG00000699 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2/25bp spacer REG00000700 [ regulatory_region (complex_region) ]
- Ci-Pitx -2751/-2696 repeated x2/50bp spacer REG00000701 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2/75bp spacer REG00000702 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x2/150bp spacer REG00000703 [ inactive_region ]
- Ci-Pitx -2751/-2696 repeated x5 REG00000695 [ regulatory_region (complex_region) ]
- Ci-Pitx -2751/-2606 delta -2693/-2646 REG00000692 [ inactive_region ]
- Ci-Pitx -5500/56 REG00000874 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -5500/-1328 REG00000875 [ regulatory_region (complex_region) ]
- Ci-Pitx -2751/-2540 REG00000873 [ regulatory_region (enhancer) ]
- REG00000876 [ regulatory_region (complex_region) ]
- REG00000877 [ regulatory_region (complex_region) ]
- Ci-Pitx -3815/55 REG00000878 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -3164/56 REG00000879 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -3429/-2250 REG00000888 [ regulatory_region (complex_region) ]
- Ci-Pitx -5500/-1328 REG00000875 [ regulatory_region (complex_region) ]
- Ci-Pitx -2760/140 REG00000885 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -3853/147 REG00000891 [ regulatory_region (extended_promoter) ]
- Ci-Pitx -3853/-553 REG00000892 [ regulatory_region (enhancer) ]
- Ci-Pitx -553/147 REG00000893 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KhL153:204692-208149|Pitx REG00001523 [ regulatory_region (enhancer) ]
- Ci-Pitx -2735/-2540 REG00000682 [ regulatory_region (enhancer) ]
CONS00001590: p.Cirobu.REG.KhL153:204692-208149>unc76::YFP
CONS00001589: p.Cirobu.REG.KhL153:204692-208149>unc76::mCherry
204,692208,149
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
|---|
Aniseed Coordinates: [204,692 / 208,149] on scaffold KhL153
3.5kb region located in Pitx first intron. Cloned upstream of the basal promoter of Fog, it recapitulates Pitx expression.
Show sequence
Length: 3,458
Cttaagtcacgcgaaatgccattctaaaacccccccctttccccccataaataccatcat
aactccaataataagatatattgtgacttataatggtttggaatccaaattcaagtgcgg
tgaaggttttatattttaaattacaccacaattttatgcttaacgacttaaattggccct
acagtaaaaactgttaattttctacctaatttgcatttttagccggtttttggataaaaa
aaactaccacaagtttcataaattagagtatgaagagtagtttaggtaaacagaagctta
aatttagcaggaaagcttatttttacatcatgtacgcaaaaacaactctgttgccggtta
aaaattcaaatcaaatatttatgacgcgctgtaatagcgtggaaaataagcggttactgt
gacgtgtaccggagcgccgcgcgcgaattaggagtcaaacaatacaagaactcgcgttta
gtttctgcaaagaggttttctgaggtccaagaactacggtttgggggtgggtgcattttt
cttgccttgttatcttggcttaagagagttattggcgcgttaaccatcgtacgtaagttg
ctggataataatacactgtccggtgcactaattattgagctccgcttcaaagctcaagta
cgttagcttatagttttgcatttaaataatagactgatgatgcaatgcaatggtgaaata
tatatctttgcacttatttgttggttaggttaatttttttcactgcaattgattggaatt
tgtcattttttggcaacaagaaaagtctaagattctaagtattagactgaattgaacgaa
tatatatcgcagtaaattgcctatctttccccttttatcgattcattaattgggtaacaa
acaaataatattaacagaattatataattgaacagtaccataaaagatcgttaattgtta
aaacatgaaaatatggaatagcaattgttttaggtatccatcttaccccacagtactata
acacaattataatgcatgagtctaagactttcgacacaatgacactctgcttattttttt
ataagaatttataaataaaacatgtaccaccttgacccatatcactaaagtttattgcaa
acaactaaaaacaattacaattaattcccaaacacagaatttcgaactcaactaaattct
agacatttttataaagaccggtttatgttacgtcatatcctgtttaatggactagaacaa
tgccattgtttttagacaatggacctttcaggcgaaaaattggaaaaatttcaacacaaa
actgttggatttaaaatgttcagaaaatttcggggttctcgtttttaaaagtaccgtgtc
aactgtcagtaaacatgcccactccttcgtttacgcaagacggttttttctaaatcctta
aaaatgccgtgagcagtcatgcaatacactacgtccaattttccggcctaaaaaggcttt
ataagtccctatgattatgaggcgtctagtaaactaaacaaactcggcaaaacgaaacac
ggaacagagcggtatttcaattaaatccgaacccaagaaccccggccgcccacaaagaaa
atccgtaggattaattccgcatggtggcgccgagtggtccgtgaaaatggaaaatcatca
cgtttttgaacgccgtttcccaagtgaatcacgttgttcagtttacatgaaactaaacgg
cggaaaagtgcggaatactgtttactgggccggaaaatctaataaaagtagatataccgc
gctaatgtggggtaaaacgtgttctttagtctggcttgttagtcatttatggttcaaaga
aaacacgagagacttatagcataggttgtacggtatgtgcctatacctcatgctcactgt
cgagttaaaagaacattggtgacatctaatgcaccaggcatacatgatgtcataccccat
atgcttatactgtggagttgagttattgagttaatacgggtgtattcttatacaccagac
acatattgcgtattcatacacctcaggctcgatgttagtttataacgtagtgtattatgt
acctcatgctcactattaaattacaacgtaactgcatttagatatttttaatacaaccgt
aagtgggcgacaaagcgctatacttaaaactttcattcgcgttttgaattttacaacatc
cgcaagttggggacaaagagttgagccataattttgcatttgcggacagccatatttccc
gctagaggcgataggaagcaaaaccttgaattcttgcagcggttcgcgcttgaagagaga
aactctgtcggctgagagtttttcaaagggactcatttgctctgtgtgtatacacggaat
gtgcttactcttacaatagtttcgggtcgctcaagccccagtgtatacgttgttactcgc
atattttataagttctatcagtttccgcagaaaaatattcagttaaaaatagaaaacaag
gatttttttgaagttttgagttttttattctgcgaggtgttaggttacatgtccagagtt
cgtgagtcgttaggtgagcgaaatggtgcccgcgtaaaagggtgccggaaatttggagct
atgttttactattgttgttgttttagttaaaaagtcgcgcttttagatctcttttaaaat
gcatatgaaccaatttctgggccgactattaaacaccaatcatttataaaaggtagattt
tacagttataaacgtattttgtcatttctaaccaatacaatttgagtgtattttggaatt
gtggttttgaatgataatttctatcagtagatgcttttatggtagttttatatgcgggta
atgctttactttacggtatatggtaacttattacagaagggggtgtataccacttacacg
tttgtttgtgctacatgtaaactctgtataaaaaatacgcgcaacgatcacatgtttaaa
gtcaaccacttggttatgacgtaataatgaggtcattcaagtttaacgaaattatgacgt
cacaattcgataaatttcattcaaatggttattgtacgtttaatttgatgattgaaggaa
ttgggattttcttaaagctctagatttaagcgactgtacgtcatcaaagggtccgaaatt
aatcgcgcttttattaattgtccgcaacgattgcaattcagcgaagacgtcacgaaaaag
gtgaactagcgatcgcctctcgctcggctcgtgtttcatatacgtcacacgattcatgag
aacgtgtgacatcagcgggggagagaccgacgcattgtttatttgaatgcagtctcttgt
agttacagaatagaacggacatacatttttatgtaaagattgtatagacagatgtatata
ctagaaatacctacaatagagaacaagttagacgacac
