Description

Hierarchy of Regulatory Regions

Regulated gene(s)

n/a

Construct(s) made to test this region

CONS00001636: p.Cisavi.REG.Ens81.R14:1337478-1337753>mCherry

Overview of functional TF binding sites in this region

1,337,4781,337,753

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [1,337,478 / 1,337,753] on scaffold R14

This 276bp region is located next to Ciona savignyi Dmbx which has no gene model in ANISEED current version. Dmbx minimal enhancer from C. savignyi was PCR-amplified with the following primers: Dmbx-Cs min F: CACTTTCCGAATCAACGTCC; Dmbx-Cs min R: TTGTCTGTCTCTGCGTACGTC.

Reference(s)

Stolfi et al. (2011). Neural tube patterning by Ephrin, FGF and Notch signaling relays. Development 138(24):5429-39