- Region 'REG00001515'
Cis-regulatory Region
Name
Cirobu.REG.KhC1.9789507-9790838|Dmbx
Short name
Dmbx −3.5 kb/−2.1 kb (Stolfi and Levine 2011)
Region ID
REG00001515
Status
Curated
Origin
natural region from C. robusta formely Ciona int. type A
Type of Activity
regulatory_region
(enhancer)
Author
Alberto Stolfi (2020-11-21)
Annotator
Marion Gueroult-Bellone (2020-11-21)
Curator
Marion Gueroult-Bellone (2020-11-21)
This shorter fragments recapitulates Dmbx expression when cloned upstream of the basal promoter Friend of GATA. Unlikely to the original 3.5kb region, it does not contain Dmbx endogenous promoter.
Modification of Cirobu.REG.KhC1.9789507-9792995|Dmbx by deletion
9,789,5079,790,838
| Motif Name | Binding Factor(s) | Sequence | Position in Region | Comment |
|---|
Aniseed Coordinates: [9,789,507 / 9,790,838] on scaffold KhC1
This enhancer is located from -3.5kb to -2.1kb upstream of Dmbx transcription start side. For double-electroporation with Dmbx reporter construct, this smaller fragment was amplified using Dmbx −3.5 kb F (CACTCATCTGCCTACATTAGC) and Dmbx −2.1 kb R (AGTACTATGACGTTACAATCC), and cloned upstream of bpFOG in order to minimize reporter construct crosstalk.
Show sequence
Length: 1,332
CACTCATCTGCCTACATTAGCTTAGTATTTTAGTTGAATAAATAAGTTCCAATATTTTCA
CAACCCGTTCTTAACTATAGAACAAGAATCGCTACGGCTCCGATGCTCGATTTCGAGTAC
CCGGTCGCCCTAGCAACCCACTTCACAATAGTAACAATGAGACCCGACCCTCCCATTGTG
AGGTAATGTTTCTTTCATTGTTACGTGATATCGAAACTCCGCCAATCGCAAGGGAAAGAA
AATCGACAGGGCACAAAATGAAATTTTTCGAAGAGTTAAAAACGATAAAAAAACAAACTA
AAAATACTAAAAGTTGTTGATTGTTATAAAAACGCGACAGAAGCGTTTATTGTTTTATCA
CAATGACGATTAACCGGACCATACTATGCCATTGAAACCGCCTAATTGAAATTTCGTGGT
CGGATTCGCTATTTTCGCACCCTGCGTTCCTCAATTTGATTGTATTCTACATCGTTAATA
TTTAATGAAATATTGACTTGTTAATCGACACTTCGCGAAAGAACAATTAAATCGTTATGA
CGTCATATGGGTAGACGATTGTTTTAATCGCGATGTTTCGACACCACCTGTTAAAATGTT
TAACGAAAGAAAATACTAAATCAAACTTTAGTACTTTAAACGGCAATTGTTTCAACTTCG
TATTCACTTACGTCTTAGGAGTTGTCTAATTTGTCAGCCATACAAAGGGGTAAAGCCAAA
AAATAATCACTCACATAGTTACATACATATGGTAACTCGTAAGCTGGCACGAGGTGTATG
AAACAGAACACTCATGTTATATACGACGTTGGTCATAACGACTGTCGTTTTCCGGCCACG
CGAGGATAAATAAGTTGCATTTACTCGGCCCATTTAGTATAAAGAAGTAAAAAACGTTTT
TATAATAAGCAAAAACTAATCAATTAAAAGAAGTTTTTAAACAGATCAGGTTGATCGTAT
TTAACCATGGATAAGAATACAATTCAGCCAATTAAAGTTTCGAATTTAAAGTTGATTTAT
TTTAATTTATTCGAATTAACAAGAAGTCGCCGATAAATCGAAGACACATTTGTGACGTCA
CGCCTTAACAATCCGCTATTGATGACGTAATAAAGGTGAATCATCTGCCATTATGAGCTC
ACAATTAACGCGTCTGATCTGCGACAAACGAAGCGCGAAATTGCTCGACGCGCTGGGAAT
AATTAACCTCGCTTTAACGCCATCTAGCGAGCACGAATTCCTGTATATTTAGGAGCGTAA
ATAACCAGAATACTCATTCGTGGTTAACAATCAGCATTGTTACGTCAATAAGGATTGTAA
CGTCATAGTACT
