Aniseed - Cis-regulation

Region 'REG00001509'

Cis-regulatory Region

Name

Cirobu.REG.KhC1:9790575-9790838_µPax3/7|Dmbx

Short name

Dmbx 264bp minimal enhancer / Pax3/7 mutated (Stolfi 2011)

Region ID

REG00001509

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

inactive_region

Author

Alberto Stolfi (2020-11-10)

Annotator

Marion Gueroult-Bellone (2020-11-10)

Curator

Marion Gueroult-Bellone (2020-11-10)

View position in WashU

Description

Mutation of the putative Pax3/7 binding site (GCGCGAAAT replaced by GGCCGAAAT) abolishes reporter activity of Dmbx minimal enhancer.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KhC1:9790575-9790838|Dmbx by substitution

Cirobu.REG.KhC1.9789507-9792995|Dmbx REG00001505 [ regulatory_region (extended_promoter) ]
- Cirobu.REG.KhC1.9789507-9790838|Dmbx REG00001515 [ regulatory_region (enhancer) ]
- Cirobu.REG.KhC1:9790575-9790838|Dmbx REG00001508 [ regulatory_region (enhancer) ]
  - Cirobu.REG.KhC1:9790575-9790838_µPax3/7|Dmbx REG00001509 [ inactive_region ]

Regulated gene(s)

KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3)

Construct(s) made to test this region

CONS00001630: p.Cirobu.REG.KhC1:9790575-9790838_µPax3/7>GFP

Show in situ data

Overview of functional TF binding sites in this region

9,790,5759,790,838

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [9,790,575 / 9,790,838] on scaffold KhC1

Dmbx minimal enhancer where Pax3/7 putative binding site was mutated (CAP letters in the sequence).

Show sequence

Length: 264

tttgtgacgtcacgccttaacaatccgctattgatgacgtaataaaggtgaatcatctgc
cattatgagctcacaattaacgcgtctgatctgcgacaaacgaagGCcgaaattgctcga
cgcgctgggaataattaacctcgctttaacgccatctagcgagcacgaattcctgtatat
ttaggagcgtaaataaccagaatactcattcgtggttaacaatcagcattgttacgtcaa
taaggattgtaacgtcatagtact

Reference(s)

Stolfi et al. (2011). Neural tube patterning by Ephrin, FGF and Notch signaling relays. Development 138(24):5429-39