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CIS-REGULATION

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  1. Region 'REG00001505'

Cis-regulatory Region

Name

Cirobu.REG.KhC1.9789507-9792995|Dmbx

Short name

Dmbx 3.2kb upstream (Stolfi)

Region ID

REG00001505

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Alberto Stolfi (2020-03-31)

Annotator

Marion Gueroult-Bellone (2020-03-31)

Curator

Marion Gueroult-Bellone (2020-03-31)

View position in WashU

Description

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.C1.1212 (DMBX1; DRGX; PITX3)

Construct(s) made to test this region

CONS00001550: p.Cirobu.REG.KhC1.9789507-9792995>unc76::Venus

CONS00001556: p.Cirobu.REG.KhC1.9789507-9792995>unc76::CFP

CONS00001574: p.Cirobu.REG.KhC1.9789507-9792995>unc76::mCherry

Show in situ data
Overview of functional TF binding sites in this region

9,789,5079,792,995

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [9,789,507 / 9,792,995] on scaffold KhC1

This cis-regulatory regions of Dmbx spans from -3,5kb to +1. It was obtained by PCR off genomic DNA template isolated from California Ciona robusta adults using the following primers (all sequences indicated as 5 to 3, from left to right): Dmbx 3.5 kb F, CACTCATCTGCCTACATTAGC and Dmbx 1start R, TATGACGTATAGAAGCAGTGG This regulatory region is active in descending decussating neurons (ddN) at larval stage in Ciona robusta and Molgula Occidentalis

Show sequence

Length: 3,489

CACTCATCTGCCTACATTAGCTTAGTATTTTAGTTGAATAAATAAGTTCCAATATTTTCA
CAACCCGTTCTTAACTATAGAACAAGAATCGCTACGGCTCCGATGCTCGATTTCGAGTAC
CCGGTCGCCCTAGCAACCCACTTCACAATAGTAACAATGAGACCCGACCCTCCCATTGTG
AGGTAATGTTTCTTTCATTGTTACGTGATATCGAAACTCCGCCAATCGCAAGGGAAAGAA
AATCGACAGGGCACAAAATGAAATTTTTCGAAGAGTTAAAAACGATAAAAAAACAAACTA
AAAATACTAAAAGTTGTTGATTGTTATAAAAACGCGACAGAAGCGTTTATTGTTTTATCA
CAATGACGATTAACCGGACCATACTATGCCATTGAAACCGCCTAATTGAAATTTCGTGGT
CGGATTCGCTATTTTCGCACCCTGCGTTCCTCAATTTGATTGTATTCTACATCGTTAATA
TTTAATGAAATATTGACTTGTTAATCGACACTTCGCGAAAGAACAATTAAATCGTTATGA
CGTCATATGGGTAGACGATTGTTTTAATCGCGATGTTTCGACACCACCTGTTAAAATGTT
TAACGAAAGAAAATACTAAATCAAACTTTAGTACTTTAAACGGCAATTGTTTCAACTTCG
TATTCACTTACGTCTTAGGAGTTGTCTAATTTGTCAGCCATACAAAGGGGTAAAGCCAAA
AAATAATCACTCACATAGTTACATACATATGGTAACTCGTAAGCTGGCACGAGGTGTATG
AAACAGAACACTCATGTTATATACGACGTTGGTCATAACGACTGTCGTTTTCCGGCCACG
CGAGGATAAATAAGTTGCATTTACTCGGCCCATTTAGTATAAAGAAGTAAAAAACGTTTT
TATAATAAGCAAAAACTAATCAATTAAAAGAAGTTTTTAAACAGATCAGGTTGATCGTAT
TTAACCATGGATAAGAATACAATTCAGCCAATTAAAGTTTCGAATTTAAAGTTGATTTAT
TTTAATTTATTCGAATTAACAAGAAGTCGCCGATAAATCGAAGACACATTTGTGACGTCA
CGCCTTAACAATCCGCTATTGATGACGTAATAAAGGTGAATCATCTGCCATTATGAGCTC
ACAATTAACGCGTCTGATCTGCGACAAACGAAGCGCGAAATTGCTCGACGCGCTGGGAAT
AATTAACCTCGCTTTAACGCCATCTAGCGAGCACGAATTCCTGTATATTTAGGAGCGTAA
ATAACCAGAATACTCATTCGTGGTTAACAATCAGCATTGTTACGTCAATAAGGATTGTAA
CGTCATAGTACTCGGCGTGTTCTGTTCTATTAAAGCGACCTCCGGTTGAACGGTAAACGC
GGATGAATTTCTCGCTAAGTTAACGACAAAATAATCAAACGTTGTTTCGATACGCGAGGC
AGGTAATAATCTGGTGTAATGAACACGCGGGTTGAGAAGGTCAGACTTGTAGAACCAAAA
CAACAGGCGGTGGTGGTATGTAGATATTTTAAATGTGCGGTGCCGTTGTATTAACAGGTT
ATGCATATTATCGTGTAGTGTAATAAGTACCAGAACTTTTGGTTTATTAAAAAAAAAAAA
CGGAATTTCGAAAAGGAGGTCAAATCCTAACCAAATCCCTGCGTCGCTATATATAACGGG
CATGTTATGGGTATTATATACTTGGGGTATGCCTATATAAAATAGAACAGCCATGCATGG
GAACAATGCAAAAGACGTGTTTGGGGTAAAAACTTTGCGTTTATCCCAATTGTACTTGCG
TGACTCGCGGTAGAAGTTTTCTCGTTACGACATATAGAGTAGAATACGGATATAGTATGA
AATAGTAGGAAAAATTTAGTGCATTTGCGCCGTTTCGTCCGCTATCCGGCAAGAGTTCAT
CAGAGAAAATGATCTCCCACATTCTCTGATTTTAAAATTTATTTAAAAAAGTTCATCGAA
AAAAGTCGTCGGAAATACACTACGTTTTTTATACCAGGCGAGTCGTTGAAAGAATATGAT
AGACCATGCTCCTGGTAGCAGAAGTAACAGATTAATATGATATTTAGGTCAGACTCGGTA
AAAGTCAGATTCAAATAACATCGAATATTTTAAAAATATGTTGCTTATAATTTCCTTATA
TAAGAACAACATGTGATTAATAAGGGCGTTGTCTGCGATTCTACGATTCCTTTTTCGAAA
AAACTCAAAAATAGTTTTGTACAGTTCTGTAGCGCATTTTTATCTGTCTACATTTTTTTT
ACGAAAAAAACTAATTTTTTTAAACAATATTTTTTGTGAGCAAAAGTTTCGTTGTCTTTC
TCGTTTTTACATCACGATACATCAGTAGTGGGCTTTAAAACTTAAGCGAAGTTTTGTCGG
GCTATCAAGTGACTTCAGGATTCTGTTAATCCCTGTTTAGACGGTAGGTAAATACATCAA
GTGTAACGTACATACGTAAGTGGCAAAAGTGATGCCAGACAAAAAAAAAAAAAACATAAT
CAAAATTTTTTAGGTAAAATGTTGTTTTATAATTCGGCATCACTGGTTTATTTTTTTTAT
TTTTCGCGAAATAAAAAAAAAATTTGTTGCTGGCAACACTGAAAGTATTATAGAGGCGGT
GATAGGATTATGACGTAACAAGGGGTGTGCAGGTGAGAAGTTGAATTTAATCCTCTTGAA
ATTTCGTTCCCGACGAATTACTGTGCGATAAACGTACTTTGGTTCAGTTAGGGAGACAGG
AACTATACATAAAACTGTTTTAATTAAGGTTTTGTTTAGTTTTTTAAGTAAGCTGTAATA
ATAAAACAGATAATTTCTTTACTACGAGTTGTCATATTAACTAATACCAGCTGCCATTAT
AGGAAACGGGTATTTAGAAGTTTAATTTTCTTGTTATGTTGCTAAATACAACGGAACTAA
TGCTTCGCGTTTCGAAAACTAGGAATAACGCTTTTTTTAGAAATTTACGATTTTTAGGTA
TATAACGTTATAAGATTAAAATAGTTCCGTGGGGTAAGATGGAACGTCTTTAGAACATAA
TATTCAAATATCTTAATCGTGTTTTAAACAATTAACAACGGTCTATATGTGATTCGTATA
AGGAAATGGTTTTAAAAATTCTTTGAATGTTCTTTGTTTATTATACCAAATGAAACGAGA
AAATAGAAAAGGTGTCCCATATTACCCCACCTTACTATGTTTTAGTGCCACGTTTTATAA
CGGCTGTTGTTTTTGGCGATTTCGTATCTCCGTTATTTACGTTTTTATAATGATATATTT
ATCCTATAACTGAAAAGAAAAGTTTAATCTAAAACGCACAAATCGTTAAAATTTGAAAAA
AAGCGCATGTGTTTTATAAAATTATGCTTTTTCAGATCTAGTACTTATTTATTTAATAAA
TTTGTTCAAATTTCAGATTTTTGCAATTTATTAAGATTAGACAAAGTTCCACTGCTTCTA
TACGTCATA

Reference(s)

Stolfi et al. (2011). Neuronal subtype specification in the spinal cord of a protovertebrate. Development 138(5):995-1004

Lowe et al. (2018). Developmental system drift in motor ganglion patterning between distantly related tunicates. Evodevo 9():18

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