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CIS-REGULATION

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  1. Region 'REG00001437' > 
  2. Region 'REG00001474'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S362:36202-36498|Lefty

Short name

n/a

Region ID

REG00001474

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

inactive_region

Author

Alicia Madgwick (2018-06-08)

Annotator

Marion Gueroult-Bellone (2018-06-08)

Curator

Marion Gueroult-Bellone (2018-06-08)

View position in WashU

Description

This 297bp sequence, located upstream of Lefty (S362.g07801), drives no reporter gene expression.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S362.g07801 (BMP10; LEFTY1; LEFTY2)

Construct(s) made to test this region

CONS00001496: p.Phmamm.REG.CG.MTP2014.S362:36202-36498>LacZ

Show in situ data
Overview of functional TF binding sites in this region

36,20236,498

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [36,202 / 36,498] on scaffold S362

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tagagcgccgtttaacgaga Reverse : tggaaaagtttgggaaccac

Show sequence

Length: 297

ttagagcgccgtttaacgagaacaactttgtggcaaatgatcccgcaggggatgcgacac
tttgcaactttgtgagatattttttgtctgcccgacgggaacgaatacacactagcgtcg
cgacggggtcgtgatttgggtttcaggagattacacttgtcacttgaagtgcgtgccgcc
tgtcagacatcaaacgctaatagtggttgttgtctacttgacaagtgccgcctatttacc
tagaagaaaattgacagacaaaacatcgtctaaatcagtggttcccaaacttttcca

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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