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CIS-REGULATION

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  1. Region 'REG00001471'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S23:115350-115650|EphrinA-c

Short name

n/a

Region ID

REG00001471

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-06-08)

Annotator

Marion Gueroult-Bellone (2018-06-08)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 300bp sequence, located upstream of EphrinA-c (S23.g00939), is active.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4)

Construct(s) made to test this region

CONS00001493: p.Phmamm.REG.CG.MTP2014.S23:115350-115650>LacZ

Show in situ data
Overview of functional TF binding sites in this region

115,350115,649

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [115,350 / 115,649] on scaffold S23

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ctgaactaagttgcgctggt Reverse : ctttgggtaaccatgagtctcta

Show sequence

Length: 300

ctgaactaagttgcgctggtattacgctgcgtacaaactaacactacaatacttggtttg
ttgacttatggacgacagactataagggcttgaggcgtcagtctgctttcagtcaaaggg
ttttaaagtctccgtcatgagtcaagttgttgaattaactgtgacgaaattaggttgacg
acctattgtttacgtttaactgcgcggaacaagatgcaacaaaacaaggcagattctgaa
cttcgtgtgacatgactacagcagagcagtacaatagtagagactcatggttacccaaag

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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