Aniseed - Cis-regulation

Region 'REG00001470'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S23:114940-115172|EphrinA-c

Short name

n/a

Region ID

REG00001470

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-06-08)

Annotator

Marion Gueroult-Bellone (2018-06-08)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 232bp sequence, located upstream of EphrinA-c (S23.g00939), is active.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S23:114940-115172|EphrinA-c REG00001470 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S23.g00939 (AL691442.1; EFNA1; EFNA4)

Construct(s) made to test this region

CONS00001492: p.Phmamm.REG.CG.MTP2014.S23:114940-115172>LacZ

Show in situ data

Overview of functional TF binding sites in this region

114,940115,171

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [114,940 / 115,171] on scaffold S23

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ggagtcgtgatgtgtatctctga Reverse : cttgacaactgcaactcctg

Show sequence

Length: 232

ggagtcgtgatgtgtatctctgatcgcagcgatagtagtgcaaaatctgtcctttgctga
gctgagctcgtacctttttctctgcgcctactttgttgtcgttctacgggagacgctcct
cggcgcagttagctgccctgacagtctttgccgatggaccaacagtaagttattgcagcc
agcccagcacaggcaagttagaattggttgaacaggagttgcagttgtcaag

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87