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CIS-REGULATION

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  1. Region 'REG00001469'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S14:101721-102143|Tolloid

Short name

n/a

Region ID

REG00001469

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

inactive_region

Author

Alicia Madgwick (2018-06-08)

Annotator

Marion Gueroult-Bellone (2018-06-08)

Curator

Marion Gueroult-Bellone (2018-06-08)

View position in WashU

Description

This 422bp sequence, located upstream of Tolloid (S14.g00600), drives no reporter gene expression.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S14.g00600 (BMP1; TLL1; TLL2)

Construct(s) made to test this region

CONS00001491: p.Phmamm.REG.CG.MTP2014.S14:101721-102143>LacZ

Show in situ data
Overview of functional TF binding sites in this region

101,721102,142

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [101,721 / 102,142] on scaffold S14

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ttgaggtatccgaaataacaaaga Reverse : tttcctggcagttgatgtga

Show sequence

Length: 422

ttgaggtatccgaaataacaaagaaccggaaaagcggtgaaaagagcgaattcttttgtc
acaatgcatccctatgacagcggagacaacagatacctttttgtgacgtaattgggagtg
tgactaccctttcaaaccgttttccccccgccactgtttttgataccgtgatgtattgga
aaaggtctgatgacgccgctggcgccaagtgtgcttgtttgttgcggcgagtgaggtcga
agcgagatccctcgaccgcatgacaaacaagggccaaattgcggtggacgggcgcctgtg
aacgccgccagggaacagtgaagtaaattggccctccgcgggaaaattgccaccgagtca
cgggcaaattaggctgtcatcaatgaccctttccctgcgcaatcacatcaactgccagga
aa

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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