Aniseed - Cis-regulation

Region 'REG00001460'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S229:72352-72621|Neurogenin

Short name

n/a

Region ID

REG00001460

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Damien Gailly (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 270bp sequence, located upstream of Neurogenin (S229_g05747), drives reporter gene expression.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S229:72352-72621|Neurogenin REG00001460 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S229.g05747 (NEUROG1; NEUROG2; NEUROG3)

Construct(s) made to test this region

CONS00001482: p.Phmamm.REG.CG.MTP2014.S229:72352-72621>LacZ

Show in situ data

Overview of functional TF binding sites in this region

72,35272,621

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [72,352 / 72,621] on scaffold S229

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : caaagtttgcacgaattcca Reverse : aaaaccacataaccgccactc

Show sequence

Length: 270

caaagtttgcacgaattccacgctgtagatcttatatagaaacacaataaagcaggcggt
ctcatgtcactgttaattaaacgcacgcccagctcctcacttcatttacctgcgctcaaa
acagacactgtatcaatagaggagttattttcacgacaggcgctacgtagaacacctgcc
gcgctgatgggattttaactgctcgacaaacacaagttggaaagctgattaagaacgcat
gttttaatcgagtggcggttatgtggtttt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87