Aniseed - Cis-regulation

Region 'REG00001459'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S229:71129-71430|Neurogenin

Short name

n/a

Region ID

REG00001459

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

inactive_region

Author

Damien Gailly (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 300bp sequence, located upstream of Neurogenin (S229_g05747), drives reporter gene expression.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S229:71129-71430|Neurogenin REG00001459 [ inactive_region ]

Regulated gene(s)

Phmamm.CG.MTP2014.S229.g05747 (NEUROG1; NEUROG2; NEUROG3)

Construct(s) made to test this region

CONS00001481: p.Phmamm.REG.CG.MTP2014.S229:71129-71430>LacZ

Show in situ data

Overview of functional TF binding sites in this region

71,12971,428

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [71,129 / 71,428] on scaffold S229

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : acacaaaagaaggccaaacg Reverse : tagctaaaggcgttaattgcag

Show sequence

Length: 300

acacaaaagaaggccaaacgtttactttctgttgggccagcttgaatcctattttggtgt
cagttctatttcaaacaaagtcttctgtataagcctgtgtcatgtgtgtctataacttgt
tttgacaggaaaatgctgtatgccatcaggtcgtaataactagcaaacataatcagctga
ctccgtggcgcaacggtagcgcatctgactccagatcagaaggttgcgtgttcaaatcac
gtcggggtcaaaattattttgtcattttaaaccaaaccctgcaattaacgcctttagcta

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87