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CIS-REGULATION

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  1. Region 'REG00001453'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S58:56959-57277|Hes-a

Short name

n/a

Region ID

REG00001453

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Damien Gailly (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 310bp sequence, located upstream of Hes-a (S58.g02084), drives reporter gene expression.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S58.g02084 (HES1; HES3; HES4)

Construct(s) made to test this region

CONS00001475: p.Phmamm.REG.CG.MTP2014.S58:56959-57277>LacZ

Show in situ data
Overview of functional TF binding sites in this region

56,95957,268

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [56,959 / 57,268] on scaffold S58

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ctaggtcgcggtcttttgtc Reverse : aaattgtaggctgccagtgc

Show sequence

Length: 310

ctaggtcgcggtcttttgtcattgaagacagtaattattggatcaagcgctttcaacgca
cctggaggcacgcgcgaaggtgacattgaccgcacagtgcacttaagcggattgtagtgc
gagtccagttgcggcagtcgggagcttttctatctaacgacagcctttagaaaatcaacg
tgaggcgacctattcgcccccagtcggtccaccgccgacattcgaccgaacaccgtggct
gacgtgttaattgtggctgatgtcggtcacgcagcatccatgacctggacgcactggcag
cctacaattt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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