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CIS-REGULATION

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  1. Molecular Tool 'Otx-MO (Imai's 25mer MO)' > 
  2. Molecular Tool 'Ci-Bra(251bp)/Ci-Sna***' > 
  3. Region 'REG00001442'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S160:19347-19784|Eph1

Short name

n/a

Region ID

REG00001442

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone (2018-05-30)

View position in WashU

Description

This 437bp sequence, located in the 6th intron of Eph1 (S160.g04451), drives reporter gene expression in neurons and sensory vesicle.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S160.g04451 (EPHA4; EPHA7; EPHB1)

Construct(s) made to test this region

CONS00001464: p.Phmamm.REG.CG.MTP2014.S160:19347-19784>LacZ

Show in situ data
Overview of functional TF binding sites in this region

19,34719,783

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [19,347 / 19,783] on scaffold S160

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tgcgtttaaaactctcagtgg Reverse : acgcaagcttgtgctcatta

Show sequence

Length: 437

tgcgtttaaaactctcagtggtttagtgcctgcggccacggtcaccagcgctaattttgg
accggtttgcaaattgcacggcgcattaaatacgccagttgtgtaatggaatgcgcttga
cgaaaaagcaaattgaatgcaagctggatcgctacacacaactggccagcaggcgcttgc
tcttaattcctaactacctttgagaatttggtcgatccctcggggaacacatcatcactc
gcgatgggatatagaccaatctgttgtcatatgtcagacaccgcaacctactagcagata
gcaaaccacaatggattattattaggtgcgcccctatagaccatcgagatggcttgcgat
aaaatgagccaagcagagttgggatgactttgctaacaaaattcgggcattaatgtttaa
tgagcacaagcttgcgt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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