Aniseed - Cis-regulation

Region 'REG00001438'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S160:16086-16385|Eph1

Short name

n/a

Region ID

REG00001438

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

inactive_region

Author

Alicia Madgwick (2018-05-30)

Annotator

Marion Gueroult-Bellone (2018-05-30)

Curator

Marion Gueroult-Bellone (2018-05-30)

View position in WashU

Description

This 300bp sequence, located upstream of Eph1 (S160.g04451), drives no reporter gene expression.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S160:16086-16385|Eph1 REG00001438 [ inactive_region ]

Regulated gene(s)

Phmamm.CG.MTP2014.S160.g04451 (EPHA4; EPHA7; EPHB1)

Construct(s) made to test this region

CONS00001460: p.Phmamm.REG.CG.MTP2014.S160:16086-16385>LacZ

Show in situ data

Overview of functional TF binding sites in this region

16,08616,385

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [16,086 / 16,385] on scaffold S160

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : aaaggggcagtttgtggac Reverse : cctcgagtcctcgacagatg

Show sequence

Length: 300

aaaggggcagtttgtggacgtgcggcgcactccgcttaattcagtggcatttctctggaa
acgcgcgccacgaggtttgtaactgcgcgtcttttgcaaagggaagatttgttattgttt
gacggtgtgtggtttacgttgaaaggaaataggcggtcgtaaagggaaagataagtgtta
gggcagaaataaatcaaaaagaggaaacagtttaattgtgtgtatatgttgcaagcatgg
taaatgtttaagttttttacgtggtgtgcttgttatttgacatctgtcgaggactcgagg

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87