Aniseed - Cis-regulation

Region 'REG00001437'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S166:44529-44800|Otx

Short name

n/a

Region ID

REG00001437

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone ()

View position in WashU

Description

This 272bp sequence, located upstream of Otx (S166.g04580), drives reporter gene expression.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S166:44529-44800|Otx REG00001437 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S166.g04580 (CRX; OTX1; OTX2)

Construct(s) made to test this region

CONS00001459: p.Phmamm.REG.CG.MTP2014.S166:44529-44800>LacZ

Show in situ data

Overview of functional TF binding sites in this region

44,52944,800

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [44,529 / 44,800] on scaffold S166

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ccgtccgctggtgtattaaa Reverse : aacgtcgtttgtttgaataatgaat

Show sequence

Length: 272

ccgtccgctggtgtattaaaatcacaccacaacatcccgctgatttgtgtccgctcgaat
gagcgcaaactactgagcatgacgtgtttgacaggcatcgtacaaagcctgacggcaacc
aaccaggctgtcgctcgcagcacgatacaaaccgccgtgagggaaaacggccagcattaa
acactgctgcttactgcacgtgactggcagaacacacctcgtttgttcgtcgaatatttt
tttaacattcattattcaaacaaacgacgttt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87