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CIS-REGULATION

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  1. Transcript 'Cisavi.CG.ENS81.R0.1...' > 
  2. EST 'AHC0AAA235YH22_RM1' > 
  3. Transcript 'Cisavi.CG.ENS81.R0.1...' > 
  4. Region 'REG00001436'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S166:45338-45692|Otx

Short name

n/a

Region ID

REG00001436

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 355bp sequence, located upstream of Otx (S166.g04580), drives reporter gene expression in muscle and nerve chord.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S166.g04580 (CRX; OTX1; OTX2)

Construct(s) made to test this region

CONS00001458: p.Phmamm.REG.CG.MTP2014.S166:45338-45692>LacZ

Show in situ data
Overview of functional TF binding sites in this region

45,33845,692

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [45,338 / 45,692] on scaffold S166

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : ctttgacctcacacgaagca Reverse : gcgctgcttacaaatttaacg

Show sequence

Length: 355

ctttgacctcacacgaagcaaaagcaattaccgatgcgtcgaaaatggaaatgaagggca
tctgtgttgcacatttgtcgtgctatgtggctgcaacaacatcagcggggcgttatgcaa
agttgttgcagtaccccgctgcttattggccggcgtgcgttctagtgtatgataaatcag
tatcgtgctgtaactggcggcccgccatgccaccaattaggtttgaggtccgcattgtga
cggagattaacacgatatgcgactagtggtcggccagagattgagctcacgtcacacacc
gcgcagcaaattgcacgtcctaaaacagtgagtccgttaaatttgtaagcagcgc

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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