Aniseed - Cis-regulation

Region 'REG00001435'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S166:46706-46976|Otx

Short name

n/a

Region ID

REG00001435

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 271bp sequence, located upstream of Otx (S166.g04580), drives reporter gene expression in endoderm, mesenchyme, nerve cord and muscle.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S166:46706-46976|Otx REG00001435 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S166.g04580 (CRX; OTX1; OTX2)

Construct(s) made to test this region

CONS00001457: p.Phmamm.REG.CG.MTP2014.S166:46706-46976>LacZ

Show in situ data

Overview of functional TF binding sites in this region

46,70646,976

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [46,706 / 46,976] on scaffold S166

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tcactgtgggttatttgtaattgg Reverse : tgccttatcgaaggggaaag

Show sequence

Length: 271

tcactgtgggttatttgtaattggtctttaacaacaagatgtaattcgcagttagtgatg
gctctcagtgcgaaggtaaacctcctttgtccctcgcttacctgctgtgtttccgctgat
atggacgttgctacatccgttcgcgccacgattcgcccagaacctcttccatatcaccga
aacactggcgtatcaggtgcgaagggaagtttggccaaactaagtgattgcacttgcatt
tagttcgaaaactttccccttcgataaggca

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87