Aniseed - Cis-regulation

Region 'REG00001432'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S166:49113-49319|Otx

Short name

n/a

Region ID

REG00001432

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

inactive_region

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 207bp sequence, located upstream of Otx (S166.g04580), is not able to drive reporter gene expression.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S166:49113-49319|Otx REG00001432 [ inactive_region ]

Regulated gene(s)

Phmamm.CG.MTP2014.S166.g04580 (CRX; OTX1; OTX2)

Construct(s) made to test this region

CONS00001454: p.Phmamm.REG.CG.MTP2014.S166:49113-49319>LacZ

Show in situ data

Overview of functional TF binding sites in this region

49,11349,319

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [49,113 / 49,319] on scaffold S166

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tccactaatctgatagtaggtgacca Reverse : tggcttcgcgtatagaactg

Show sequence

Length: 207

tccactaatctgatagtaggtgaccattaagtatatagtccacccagtaatgcgcaccta
tacatcgaactatcccgtgataatttctcctggcgtgtgcacctcctcgcctcattatca
catagcgccgtgctttgtgacgtttgtttacttttgagccacgcaagatgcaccggtttt
ccacggccagttctatacgcgaagcca

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87