Aniseed - Cis-regulation

Region 'REG00001430'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S1711:9716-10015|Nodal

Short name

n/a

Region ID

REG00001430

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 300bp sequence, located upstream of Nodal (S1711.g15500), drives reporter gene expression in mesenchyme.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S1711:9716-10015|Nodal REG00001430 [ regulatory_region (enhancer) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL)

Construct(s) made to test this region

CONS00001452: p.Phmamm.REG.CG.MTP2014.S1711:9716-10015>LacZ

Show in situ data

Overview of functional TF binding sites in this region

9,71610,015

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [9,716 / 10,015] on scaffold S1711

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tcaaacttaaaacgtaaacttgcat Reverse : aaaagcgacccagctgaac

Show sequence

Length: 300

aaaagcgacccagctgaacacaggtgcaaaggtttctttaacacgttgagttcaaataaa
ccggaacataagacaaagtttaacgatcaaacattttggttgtggtaatgcttatccagg
tttccttccgaagtgtgactttcttgtcgcttgtgcaaatactgcatctgacagatgatg
gtgtttgaacttatcggtgtctacgcgtgctgctttctatgcaaataaattagtgcatcg
gaggtgattactttaccaaaagttgtcagagtaaaatgcaagtttacgttttaagtttga

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87