Description

This 290bp sequence, located upstream of Nodal (S1711.g15500), drives reporter gene expression in muscle and nerve cord precursors at early gastrula stage.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S1711.g15500 (BMP10; GDF6; NODAL)

Construct(s) made to test this region

CONS00001451: p.Phmamm.REG.CG.MTP2014.S1711:9130-9419>LacZ

Overview of functional TF binding sites in this region

9,1309,419

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [9,130 / 9,419] on scaffold S1711

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : cagacgtggtgtgaattatagagg Reverse : tttcgtaaaatatgttaacttccatgt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87