Aniseed - Cis-regulation

Region 'REG00001426'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S2332:10795-11099|Lhx3/4

Short name

n/a

Region ID

REG00001426

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(extended_promoter)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 306bp sequence, located in an intronic region of Lhx3/4 (S2332.g16546), drives reporter gene expression in endoderm and mesenchyme.

Hierarchy of Regulatory Regions

Phmamm.REG.CG.MTP2014.S2332:10795-11099|Lhx3/4 REG00001426 [ regulatory_region (extended_promoter) ]

Regulated gene(s)

Phmamm.CG.MTP2014.S2332.g16546 (LHX1; LHX3; LHX4)

Construct(s) made to test this region

CONS00001448: p.Phmamm.REG.CG.MTP2014.S2332:10795-11099>LacZ

Show in situ data

Overview of functional TF binding sites in this region

10,79511,100

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [10,795 / 11,100] on scaffold S2332

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tattttgcgtttggaaatttaac Reverse : cagaaaagcattttgctggtg

Show sequence

Length: 306

cagaaaagcattttgctggtggtttatatattcgaacacgcactgtccgtcctttttccg
tttcgcctacttaaaagttgtactggaacctcgtggggagtaaataaacaaggaaggtgt
cactgggcacacaaacaaacaaattagttgtaatcaatgaagtaagcggatgtcgctgaa
cttctgacgagtacctaacgttactttgtttcatacacgaatcaaacagtgccgttcacc
taacaaatatttgcacatgttttatgtgaagcgcggtggataagttaaatttccaaacgc
aaaata

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87