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CIS-REGULATION

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  1. Region 'REG00001425'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S2332:6677-7993|Lhx3/4

Short name

n/a

Region ID

REG00001425

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-29)

Annotator

Marion Gueroult-Bellone (2018-05-29)

Curator

Marion Gueroult-Bellone (2018-05-29)

View position in WashU

Description

This 1316bp sequence, located downstream of Lhx3/4 (S2332.g16546), drives reporter gene expression in mesenchyme.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S2332.g16546 (LHX1; LHX3; LHX4)

Construct(s) made to test this region

CONS00001447: p.Phmamm.REG.CG.MTP2014.S2332:6677-7993>LacZ

Show in situ data
Overview of functional TF binding sites in this region

6,6777,992

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [6,677 / 7,992] on scaffold S2332

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : aacctaagctcgatcagtcca Reverse : cgtcgtatgtgtcaaaactgc

Show sequence

Length: 1,316

aacctaagctcgatcagtccagattacttttacagcccccaccatagtgggtttaaacgt
tcgccaaaccttcagaaactatagcctaccacaagtgattaactgatttacaattgtggc
agttcaatgcagagcgggtggtaccactatgaataggctatgagccaaactgtattcgcg
ataattacctcaatatggacaaaaattgtgcgaagtagtcaattaaattgggaaataaaa
atatttgcaacttttattctgcatattataaaagaaaatggctcactgaattttggtgga
tgtttctagtgagcctacacccgtcgcctcccggaattcagctcgtcctgcctgggggcc
aaagggccctgtttgagaaccactgtactaagaagttttttggtgatgagaagtcacctg
ttttcgcccagcagtattatgtggttttcttaaattagcatgcgcattttcagtgttgta
aatgtttccgttcgttttgtacaatttttgtaaacatatttacttttacattttaaccac
tgtggttttacttgcaaaacagtcgtgttagtaggtcaaatggtctgtatagaaaatcag
taaattggatatagaatatatatacggaaatatataggaaagtcagtacctttctagttg
cattattgtcatacttgcattgcattgaatcacccgtttattcgcgtactaatccctgat
ttgggaagtaaaaaccccgggtacagagttgtaaacggtcctgaaattggagcccgaacc
tggcccgtacccgaaagtacaagtccgaaacccggccctaatccgaatacattgaagccc
caggaaagaccaagacaacaaacacaatagacaataatagacaattgaaacgttttgggg
ctgttttaaacaaggcgttttggggacattatgtcaggtccatcaactagctacttagct
gtggaagcctgatcccgggattttcccatttagtcccgaaatcccagccaataggaatga
agcctttagggctgatcgcaatgaatatctcacgaaaagtttagtttcgatgtcaaatcg
ttgtttcattggtatgtaggttttcagttattcaacttttaaccaaacatgtctataaaa
cgtgtttaagtaaatgctcgaaatgctgcaacactagtttaatcgaacgactaaaacgta
ggcaacgtgtactttttaccgtgtgaagaaataagccagtgaaacttttttactgtatgc
gataatattttatagtcaaataaacgataaatcttgcagttttgacacatacgacg

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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