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CIS-REGULATION

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  1. Transcript 'Cisavi.CG.ENS81.R51....' > 
  2. Transcript 'KH2012:KH.C3.312.v1....' > 
  3. Molecular Tool 'En>Ngn::WRPW (Stolfi 2011)' > 
  4. Region 'REG00001421'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S253:122003-122412|FoxD

Short name

n/a

Region ID

REG00001421

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-28)

Annotator

Marion Gueroult-Bellone (2018-05-28)

Curator

Marion Gueroult-Bellone (2018-05-28)

View position in WashU

Description

This 410bp sequence, located upstream of FoxD (S253.g06179), drives reporter gene expression in endoderm and trunk latteral cells.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S253.g06179 (FOXD1; FOXD3; FOXD4)

Construct(s) made to test this region

CONS00001443: p.Phmamm.REG.CG.MTP2014.S253:122003-122412>LacZ

Show in situ data
Overview of functional TF binding sites in this region

122,003122,412

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [122,003 / 122,412] on scaffold S253

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : tgagaaactaggacaccaaaagc Reverse : ccaaaaatgatattgagactttgc

Show sequence

Length: 410

tgagaaactaggacaccaaaagcagtgggaaaaggcttaaaacacaatgtccaatgttca
attccaagcgcgccgtatggttaataagttgccgttacaagccattgaacagttgtttcg
catattagcgctgtgtagcgatgtttcatggcaaacaacgccagtcgaagcgttgagcgt
caaatcagccataaattgtcattccctttctcgcaaactctctccgcaaaagcccgaagt
cgaagctccgaaaagcaaacccgacattagcgttagacggctccgcgagtcgggtttttc
aaagtaagatggccgcgtcgcgccaggctaaactgtggacgttttgaggcgttaaagtat
acgtttttttctaaacatatttagttgcaaagtctcaatatcatttttgg

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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