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CIS-REGULATION

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  1. Clone 'cilv041k04' > 
  2. Region 'REG00001411'

Cis-regulatory Region

Name

Phmamm.REG.CG.MTP2014.S128:57500-58015|Fgf9/16/20

Short name

n/a

Region ID

REG00001411

Status

Curated

Origin

natural region from P.mammillata

Type of Activity

regulatory_region
(enhancer)

Author

Alicia Madgwick (2018-05-28)

Annotator

Marion Gueroult-Bellone (2018-05-28)

Curator

Marion Gueroult-Bellone (2018-05-28)

View position in WashU

Description

This 516bp sequence, located upstream of Fgf9/16/20 (S128.g03805), drives reporter gene expression in endoderm, TLCs, mesenchyme, notochord, TVCs, muscle, nerve cord and sensory vesicle territories.

Hierarchy of Regulatory Regions

Regulated gene(s)

Phmamm.CG.MTP2014.S128.g03805 (FGF16; FGF20; FGF9)

Construct(s) made to test this region

CONS00001433: p.Phmamm.REG.CG.MTP2014.S128:57500-58015>LacZ

Show in situ data
Overview of functional TF binding sites in this region

57,50058,015

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [57,500 / 58,015] on scaffold S128

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : gggccacagtgtcaaaatta Reverse : ttggtgcaacctgatctgaa

Show sequence

Length: 516

gggccacagtgtcaaaattatggtttttgcgcatatgcatgtggggcatcgttggtgcca
cgtatattttgattgacagccggtgcgcttgacgaggcgtcgtgcagtgctcgtgggcga
aagtgcaatgtggagaattcgctaatatttgccgtttaatcccgaacgcatgcggtcaga
aggcgatacacagagattaaaaatctaatgtatacaatgcaacaatagcagcgggtgagc
gagaaaaagggcgaatcgtgccctataccgacgaggtcttcgccaggtcttttaatgaga
cgtcggccgctgtgcagaaaaaagttgacgagtgggcagcttgaaaaagtgtgcgttcac
cgaaagcgaagtcgcttccttttattatcgtgccccaatcatggaaagtcacagatcagt
cgtgaacgctcatggcgttcccaacttcatgtgaaatcaatggaatgataatgagaaatt
agaaatgtatgtgtcattcagatcaggttgcaccaa

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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