Aniseed - Cis-regulation

Region 'REG00001493'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L152:100571-101109|Islet

Short name

Islet -5915/-5396 (Stolfi 2010)

Region ID

REG00001493

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Alberto Stolfi (2019-02-26)

Annotator

Marion Gueroult-Bellone (2019-02-26)

Curator

Marion Gueroult-Bellone (2019-02-26)

View position in WashU

Description

This cis-regulatory sequence drives reporter expression in bipolar tail neurons only.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L152:100246-101451|Islet by deletion

Cirobu.REG.KH2012.L152:99298-106485|Islet REG00001488 [ regulatory_region (complex_region) ]
- Cirobu.REG.KH2012.L152:102537-106485|Islet REG00001489 [ regulatory_region (enhancer) ]
- Cirobu.REG.KH2012.L152:99298-102536|Islet REG00001490 [ regulatory_region (complex_region) ]

Regulated gene(s)

KH2012:KH.L152.2 (ISL1; ISL2; LHX9)

Construct(s) made to test this region

CONS00001557: p.Cirobu.REG.KH2012.L152:100571-101109>unc76::CFP

CONS00001520: p.Cirobu.REG.KH2012.L152:100571-101109>unc76::mCherry

CONS00001519: p.Cirobu.REG.KH2012.L152:100571-101109>unc76::GFP

Show in situ data

Overview of functional TF binding sites in this region

100,571101,109

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [100,571 / 101,109] on scaffold KhL152

This 538bp sequence was given by the author. This cis-regulatory region is located upstream of Islet (KH.L152.2) TSS (-5915/-5396bp).

Show sequence

Length: 539

attttgtgctaaccgtattcatatttgcaacccactgtgagttgcttcttattaggttta
acagccacggctttaactaaattttatttattttaaccttagcgtgttataacgactgtt
atttttttgttaattcccatttcttcgccatgttagttaatatgacttttgttattgtaa
ttatataaaaatatatttttaaaataagtctaaaagtaaattccaaccaagttgtattta
caaatttccgcaccagaataggcagcaggcaaccgcggatcgaaacaagtccctgattta
tggttttgttgcaactaattatggctgtacagtacacaggtcccaggattgccgtaaaac
ttaatttgctggaatatgttgaacaacagttgttacactctgttgctatattaagtttag
cggccacgctttttttaagccttttattccagtattttcgctcgtagcgtgttgataacg
actgttttgctgctaattctcttcgttgttttaggtaaacttcgctatcgtattcaaaa

Reference(s)

Stolfi et al. (2015). Migratory neuronal progenitors arise from the neural plate borders in tunicates. Nature 527(7578):371-4

Stolfi et al. (2010). Early chordate origins of the vertebrate second heart field. Science 329(5991):565-8

Stolfi et al. (2012). Genetic and genomic toolbox of the chordate Ciona intestinalis. Genetics 192(1):55-66