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CIS-REGULATION

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  1. Region 'REG00001490'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L152:99298-102536|Islet

Short name

Islet -7216/-3950 (Stolfi 2010)

Region ID

REG00001490

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(complex_region)

Author

Alberto Stolfi (2019-02-26)

Annotator

Marion Gueroult-Bellone (2019-02-26)

Curator

Marion Gueroult-Bellone (2019-02-26)

View position in WashU

Description

This 4.2kb region located upstream of Islet (KH:L152.2) drive strong reporter expression in atrial siphon muscle precursors, bipolar tail neurons and cholinergic neurons of the central nervous system. Compared to the larger 7.2kb fragment, it lost the ability to drive reporter expression in the notochord.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L152:99298-106485|Islet by deletion

Regulated gene(s)

KH2012:KH.L152.2 (ISL1; ISL2; LHX9)

Construct(s) made to test this region

CONS00001577: p.Cirobu.REG.KH2012.L152:99298-102536>unc76::mCherry

CONS00001578: p.Cirobu.REG.KH2012.L152:99298-102536>unc76::CFP

CONS00001521: p.Cirobu.REG.KH2012.L152:99298-102536>GFP

CONS00001518: p.Cirobu.REG.KH2012.L152:99298-102536>unc76::GFP

CONS00001522: p.Cirobu.REG.KH2012.L152:99298-102536>H2B:mCherry

Show in situ data
Overview of functional TF binding sites in this region

99,298102,536

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [99,298 / 102,536] on scaffold KhL152

Primers used to amplify this cis-regultory region : AscI Islet -7216 fwd (tatggcgcgccCAAGTATACCACGCGAGTTAG), (XhoI) Islet -3950 rev (tatCTCGAGACTCAATTCAACGG).

Show sequence

Length: 3,239

caagtataccacgcgagttagttcggccaggaagcgcgctgtttattatcaagttattat
gacgtcatataaaatacgtcacatttgtagcacctggttatctgccttcccacggttaaa
acagatgcgacctcggttatcagtggtttgacatggagatcgtaaatcaagcgacgaata
tctgtgacacgtcacgtgacgagattgctgacataccaggacacttaacggtatctggtt
aggggaagtacctgtagcgtcatacacataacctgcataatgctttatagcttcgaatac
gatagcgaagatcaaattaattaaaacaacgaagagaaggattcgaagcaaaacgacagt
cgttaaaacacactacgggtaaaaattacttggggaaaagcgtggctgttacatccaaat
caaaatgtaagccgtcaatggtttgtgaacaaacggtaactcttgtcacctctaagctaa
actcattttttgcagagtttaaaaataccgagtagcaactgccgtctggaacgaataaat
gaatctaacgacgcagacgttatgacaggtgtgttcatacaccatgtttcccttttacgg
gaaagcgtataaaatatatatttacattcttgcctaaagtataaacgaacttttattgaa
tggtcaaaatgggtcatcttaaattccattataacttttccatttcaaacatttttccgc
agcatcgtcataaaacagggattccaatagaaacatcataaaacaccaaaccatgttgtt
tatggatcaccgtgtttgttgtattaacaagagttataactacaacatgacgtgactaga
ttgacacaacaactatggttggcagtcaaaacacacgaccccaccccataagatggagcc
acgccaccaaatcaacacagcagaagctccaggtggtttggtaggttctgttatcacaac
actacaggccatgagatctatgatctataccgtctgttatatttagtggatttgtgattt
taaccgaacaccttcactagtttaaaccaaccgacaactcaccgcgaacaatcagcgaat
atttcattaaaccgacaaaaatttccagttaatttaaaatatagtaagttggggtaagat
gggacatgtttttattctcatctcgtcccatttggtagtcaacaaagaatatttacagaa
ttataaattgaaatatcttcacgaagagaaaaatattgttatttgttaaaaacacgatca
ggaaatatgggatattttgtgctaaccgtattcatatttgcaacccactgtgagttgctt
cttataaggtttaacagccacggttttaactaaattttatttattttaaccttagcgtgt
tataacgactgttatttttctgttaattcccatttcttcgccatgttagttaatatgact
tttgttattgtaattatataaaaatatattttttaaaataagtctaaaagtaaatgtcta
agttgtatttacaaatttccgcaccagaataggcagcaggcaaccgcggatcgaaacaag
tccctgatttatggttttgttgcaactaattatggctgtacagcacacaggtcccaggat
tgccgtaaaacttaatttgctggaatatgttgaacaacagttgttacattctgttgtttt
attaagttcagcggccacgcttttttaagccttttattccagtattttcgctcgtagcgt
gttgataacgactgttgttttgctgctaattctcttcgttgttttaattaaacttcgcta
tcgtattcaaaatatgttcatttagttggtaggttatgtgtgtaagtgacatcataacaa
cttgtatgaggcaaacaaggggttatgacgtaataatagaattattgatgtttcacaggg
tgaggtgtgacgtaataatagttctgacagttaaagtgattttgaataaaaacaaaaaac
agaatgtttaagtcgcgaaaatcgcgtgggcagcgttaaccagagcatcgcatgtgacaa
gcgaacgaaatcccagcagatctacgacgttggtccgctgagagttcgagcgaattcggg
atgtaacgtcgatgaaagacgaaaagcggtccttgcctcgacaagaaaggacgaaccgcg
cgatagtagattttatggcgtatggtgttctataacacagctgtgggttgaagtggtttc
gaatatttaagcaaggtacgacttgaccggttacgtcactttgaccacgtggtcagctct
catcaatatttattttaaatttgattaaacagcgacctcaggtgagcggcctaattatgc
gcctctatgacgtcatagtttaaacttcttttaatagcggaatcgaagataaataggttg
cgttaccctgggggtcgagatccaaatttcgttacttcgattgtttaattgattcgacat
cgagttaaagcgcgagatttgattacatttccattgttcaatccataattcgctttgtga
cgtaacacaaacttaacaaacacgtggtagcgcgcaagagctaattttttaaacgtttct
tgattttttttcgattttttttattaaaaaataaaataaattaatattttgttgaaatac
gtcgaaaccgcgaaaacgcgatcgacgcaattcaatttgtaaatgaaatatcgaccagtg
gcgccgatagcggataatcctcacaattcttgtgataaattagtttcttactcggataat
ctgtgtgccggtttgattgtcgcacccaaggtgattggggggggggggatattacgtcat
tatgacaaataatttcgattaaaattggtaaaaatataaattaggggagtttttagtttt
gttttaaaagattttatttaaaaattaaaaaaaaaatcgaaaatttttgaatttgatcga
ttagctgtaattacgtcatcaatccgcttattatgacgtcatacgtcgccatattgcgtc
ataatcgggcgtcagaatgtcgtcacaaaccgtattgtgttgaattaattggaattatga
aacgccgctcggtcgggggatttgtgacgtcacaaacgtgaatgttacgtcacaaacggc
gaactcgggcgaacttttagctttgcggataataagggtaagtgacgcaacaatgacgca
acaatgttgtcccttgtgacgtaacagagtgtattgtaaccgttgaattgagtctcgag

Reference(s)

Stolfi et al. (2011). Neuronal subtype specification in the spinal cord of a protovertebrate. Development 138(5):995-1004

Stolfi et al. (2010). Early chordate origins of the vertebrate second heart field. Science 329(5991):565-8

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