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CIS-REGULATION

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  1. Region 'REG00001489'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L152:102537-106485|Islet

Short name

Islet -3950/+1 (Stolfi 2010)

Region ID

REG00001489

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(enhancer)

Author

Alberto Stolfi (2019-02-26)

Annotator

Marion Gueroult-Bellone (2019-02-26)

Curator

Marion Gueroult-Bellone (2019-02-26)

View position in WashU

Description

This 3.2kb deletion causes the loss of the enhancer activity in atrial siphon muscle precursors, bipolar tail neurons and cholinergic neurons of the central nervous system.

Hierarchy of Regulatory Regions

Modification of Cirobu.REG.KH2012.L152:99298-106485|Islet by deletion

Regulated gene(s)

KH2012:KH.L152.2 (ISL1; ISL2; LHX9)

Construct(s) made to test this region

CONS00001652: p.Cirobu.REG.KH2012.L152:102537-106485>unc76::GFP

Show in situ data
Overview of functional TF binding sites in this region

102,537106,485

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [102,537 / 106,485] on scaffold KhL152

The 4kb region upstream of Islet (KH:L152.2) TSS drive strong reporter expression in notochord.

Show sequence

Length: 3,949

ggacaaaagttgggaaggaacgcagcggttgttgttggttggtatgctaccatggtagtt
gtgtgtgcaatgtatggctattgtgggtgtatgtaagtatgtatagttgggtgtattgta
tatgtttaatgtttgggtgtggttgtggcactatacttatatatagtagggtggggaaga
tgggacacctttagcacataacaattaacaacggtctatgggagtcgtggaaatacggtt
ttatagttctttgaatgttctttgtttagtatcaaatgggagaagaatatagaataaaaa
ggtgtcccatcttaccccaccctactatatatggcgtatggttggctgggtagtagtttt
gtgtgttgggtatacatgtgtgtgtgtggtgttggttgtggcgaacttgtatttatccct
gttgatgcttaagaaccaccgtcgttttttcactaaatattcaaaatcacaaacaatctt
tttttattaagactttgccagcatttcgattttttcctcatatgagagaaagcgactcaa
acgtcacttggtcatccattatgcatgagggccggcatgcgggggaaaaatcgacatttt
tgacgtcataaataacccgaagtttgaaccgcgggttgaatatcccgcctcgtatccggt
gatatagtagagtgggggcaagatgagacaccctttccattctgtttttgtcaaacaaag
aacatttaaagaattataaaaccgtatcctcacggctcccattgaccgttgttgattgtt
taaaacacgatcgtggtatttggatattatgtgctattcgtgtcccatcttaccccacag
taagttacatgtttagcaatcaagttaaacgactttgttgttgaagttgtgtttgttaaa
acggatgagcggagggcaatgaacataaaaataacataaagtataaagtagttccggaaa
tcgcgcgtttttactgtttaaatcaattttacaatgcgcagaatagtattattatgacgt
aataaagttacgcgagggttaaagcgacgagatttgtcgaacgcgagaataaaagatctg
aagcgacgccgagagaaaaaagaaaaaagcgaaaatcttggtggcagtaaactgtccagg
gttgcatgaacagcatgctgtgactgtagtggttggacgtgctggtgggtttgggggtta
ttaaggggtagtgtgtatgacgtaataatgcaggtagtgacgtcagtatagtaaggttga
ggtcaaaagttaaaaaattaacaacacgtgttagtggttgttgtatggggtttgaggttt
tatttaatttaatattaatattgttgctgtttttgttgtttaaaagttagttggattatt
ttgttgattgtgcgaaataattgttaaaagatttgaattaacaaaatggcgttttcgcga
acgcgcgttgagagggggtatttacaaatgacagattgtcaagcatcgcatgaggtttaa
acatcgcatacaatgtccaagccccgtaaaataatgtgtttgtgaaaaagtacaaaaata
attttaaattttttttatcaaatttcggccgggtcaatttttttcgaaaagtttcaagtt
ttttggaatttgtagcagagtgagctcgcttgagttattgctaacaaggcgtggagtcag
ctttcaacaacttcacttggaagacctccagttatggatggttgaacgaaacttatcgac
caaaaaatgattcaaaaatttagaaattatttaaaactttaaaaatttcgctgcagaaat
aaagcaagaattagctggttggtccgttgggttccttagttaaagatctggcagtctgcc
agggccggcgtcggcaaaataaaatatctacaaagcgagctaccgacagggtaactggag
accataactttcaattcggccattttcaacaaagcgacttgacaacttaaactttaaaag
ttcaaaaaaaacgaacggattcttaaattttaacaacttgttcttgaatattcaattctt
ggaatatttctcgcgtcgttctagaattattctgttcgcattgtgacgtcataatattgt
tttgtcagcccattgtcagttttcgcttcaatgaatccacgtgcgctgtataataatggg
gattgtgacgttgtttgtgttgtttgtttgaaataaactttattgtgacgtaacacgtat
gagaagatgttgtttgttatgtcatatttttcttaactctgtataatgacgtcacaatcg
ctggattgttgcgaaattctagaattttgatcttttcgtcgaatcgtgaccgcaagaaag
cgaaggaaattcattcatgaaattcggggtaaaaatgccgattgtgacgtaatgaatcct
tggctcgacatcgcgattcatctaagtgacgtcacaataggttgcggtcgtagcatcggc
ggcatccagagttcccacgacccccagcttccttgctagtggtttattcacattcactaa
tcttattaacaatgaaatcgcaacgaattaataatcgttaatctcgtgttgctgccgtta
aatagctacgctcgcgacgtcgactctcaaactaaaatggtggtcgcgcgtacagcgtgt
cgcgcgctatatttgtgacgtgtgtatgatgtaataaagaaaggttttgttaagttgaat
tccagaacaagttgaatattataattaattgttacgtcataaagatcgggtgctttggac
attgttcgccgagattgaaagcgacgaaggcggcgcggtttgttcccgatgtcgcgacat
gtcgctgtcattgttgagtgacaatagaagacaattaaaccgattgtcacaaatcgcgat
gtccaattgtcacgtgaatgaatttttaattttactcaaattttgataaatcatgacatc
atatttttatatcgcgtcacttattttattacattataaagcgcgtattatgacgtcaca
ggcgtatggcgttgcaaatattctagcagcataataattcattattatgtaacaatgcga
ttgttacaacgtcgttctggcaattgtcgccttctgttacgtcataatgaagtcgtcttg
cacgcggtggggacgataaatgtttcatggggcgaatgcttcggcgcaactcgtttgcag
ccgaacgtaaacacaggaaggtgtagggcgatgcgtgcgttgggtttggtgaaatatatt
tgttaaagttggtatttaacgactcatatagtttaaagacgttttatattttcgattttt
tgcctgaaattcggcaaaacttgatcagagataaaaaaaagttggaaaaagaacgttttc
atacctagatgagccgctgaaaaatgaattaacattatgcctggtagcaaaagtaaagga
agataattgtctgtttggatttggtaaatattggtaatgttatacgcgtgtggggaggtt
aattaacatgggcgtgtgaaatgtaattagttaatgtagttagtcgtatctggatcggaa
ggcattcatttgcactgtaggcattgaactaggaggttcaccacatcgaggaatatttgg
tcgcgctgcggggattgagggccttgtagttggggggaggtttgaaagtttcccgaagta
atattaactttgttgtataggacccgttgttgctgttagagagaatcggagaaaaagttt
cgagagaagctttagataaattgattctgtcgcattcgagtattacaacttgtgtctgtg
acgtgagcgagtgaggacaatttactgtgacgtcataatagccagcaatataggggtgga
aggtgaataccccgctctacaagcggagaatttcgaaatttcaaacctggatcccggata
ctcgccgccgttggcggacgactcaaggtcggggtcgttcacaagtttt

Reference(s)

Stolfi et al. (2010). Early chordate origins of the vertebrate second heart field. Science 329(5991):565-8

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