Aniseed - Cis-regulation

Region 'REG00001408'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.L106:34858-35128|Nodal

Short name

n/a

Region ID

REG00001408

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Alicia Madgwick (2018-05-28)

Annotator

Marion Gueroult-Bellone (2018-05-28)

Curator

Marion Gueroult-Bellone (2018-05-28)

View position in WashU

Description

This 271bp sequence, located upstream of Nodal (KH.L106.16), drives weak reporter gene expression in muscle.

Hierarchy of Regulatory Regions

Cirobu.REG.KH2012.L106:34858-35128|Nodal REG00001408 [ regulatory_region (extended_promoter) ]

Regulated gene(s)

KH2012:KH.L106.16 (BMP10; GDF2; NODAL)

Construct(s) made to test this region

CONS00001430: p.Cirobu.REG.KH2012.L106:34858-35128>LacZ

Show in situ data

Overview of functional TF binding sites in this region

34,85835,128

	Motif Name	Binding Factor(s)	Sequence	Position in Region	Comment

Sequence

Aniseed Coordinates: [34,858 / 35,128] on scaffold KhL106

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : cccacaaatgaaatgcaaat Reverse : tgacgtagctagcaatcaactg

Show sequence

Length: 271

cccacaaatgaaatgcaaatattcaagttttcttaagtagcgattgatatttgatgtgtt
gtttattcttgagccttacgttagtttgaaagcgaggttggcgtgcgttcgtcgctttga
tggaaggtgttagcttgctgcccgttcaaccaacgcaggtgtcccacgccctcaagcttt
ttcgagagaaaaaagatcccctcacctcgtatataaacccgcgcactcggtgaatcagaa
tcacaatcgcagttgattgctagctacgtca

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87