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CIS-REGULATION

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  1. Region 'REG00001404'

Cis-regulatory Region

Name

Cirobu.REG.KH2012.S215.4:99820-100244|Lhx3

Short name

Cirobu.Lhx3. intronic 425bp

Region ID

REG00001404

Status

Curated

Origin

natural region from C. robusta formely Ciona int. type A

Type of Activity

regulatory_region
(extended_promoter)

Author

Alicia Madgwick ()

Annotator

Marion Gueroult-Bellone (2018-05-28)

Curator

Marion Gueroult-Bellone (2018-05-28)

View position in WashU

Description

This 425bp sequence, located in an intronic region of Lhx3 (KH.S215.4), drives reporter gene expression in endoderm, mesenchyme, notochord and muscle.

Hierarchy of Regulatory Regions

Regulated gene(s)

KH2012:KH.S215.4 (LHX3; LHX4; LHX5)

Construct(s) made to test this region

CONS00001426: Contstruct Alicia TEST

CONS00001425: p.Cirobu.REG.KH2012.S215.4:99820-100244>LacZ

Show in situ data
Overview of functional TF binding sites in this region

99,820100,244

Motif Name Binding Factor(s) Sequence Position in Region Comment

Sequence

Aniseed Coordinates: [99,820 / 100,244] on scaffold KhS215

This sequence was retrieved using ANISEED Blast tool. Primer used for cloning : Forward : TCGTTACAACACGCTTAACTACTAA Reverse : ACGTCGCGCAGGTTTATATATTCT

Show sequence

Length: 425

tcgttacaacacgcttaactactaactaatattgtcaaatatttgtttagtaatgaatta
cattgttacgtcataatgttccggttcaaaccgaccaatcacagcttgatcttcaaacaa
aacataatacgtaatttccgaatgacgtcactttgaacgacgtcacgccgacacatcaca
aatgcaaataccaaaacggatactacgtacttgtgacgtacacctgacgtcatccttctc
gctgcgtcgtctgtaccgcattatgacgtcatgagaaacaccgctgtttgcattgtgacg
tctagatgttacgtcatcaaagcggcgtaattcgtttgttaagcgcgatatcccgctgat
caaagcgaggcgttaattgtttaagaaagaaagtggattctagaatatataaacctgcgc
gacgt

Reference(s)

Madgwick et al. (2019). Evolution of embryonic cis-regulatory landscapes between divergent Phallusia and Ciona ascidians. Dev. Biol. 448(2):71-87

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